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GAPDH antibody

Anti-GAPDH antibody used in Western Blot (WB). GTX85118

Cat. No. GTX85118

Reference 2

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all GAPDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human GAPDH.The immunogen is located within amino acids 60 - 110 of GAPDH.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726255

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

Cellular Localization

Cytoplasm, cytosol,Nucleus,Membrane,Cytoplasm, cytoskeleton

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody used in Western Blot (WB). GTX85118

GTX85118 WB Image

WB analysis of HeLa cell lysate using GTX85118 GAPDH antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

Application Reference

Beauchemin M et al. Pharmacol Res 2020; 152 : 104589 Exploring mechanisms of increased cardiovascular disease risk with antipsychotic medications: Risperidone alters the cardiac proteomic signature in mice.
Application : WB
Reactivity : Mouse
Baumann J et al. Mol Biol Cell 2017; (Epub) Golgi stress-induced transcriptional changes mediated by MAPK signaling and three ETS transcription factors regulate MCL1 splicing.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399