WB analysis of 40 ug of HeLa nuclear fraction (NF) (Lane 1), HEK293T control (Lane 2), and HEK293T GATA4 over-expression lysate (Lane 3) using GTX54530 GATA4 antibody.
Dilution : 1:1000

FACS analysis of Hela cells using GTX54530 GATA4 antibody compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

ICC/IF analysis of permeabilized embryoid body endoderm generated from human episomal iPSC using GTX54530 GATA4 antibody.
Green : Primary antibody
Blue : cell nucleus
Dilution : 1:100 in 3% BSA/PBS blocking buffer overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

IHC-P analysis of human pancreas tissue using GTX54530 GATA4 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

WB analysis of whole cell extracts (20 μg lysate) of SH-SY5Y (Lane 1) and Hep G2 (Lane 2) using GTX54530 GATA4 antibody.
Dilution : 1:500-1:2000

ICC/IF analysis of HeLa cells using GTX54530 GATA4 antibody.
Green : Primary antibody
Blue : cell nucleus
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for 1 hour at room temperature

ICC/IF analysis of HepG2 cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

ICC/IF analysis of NIH-3T3 cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

IHC-P analysis of human small intestine using GTX54530 GATA4 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

FACS analysis of HepG2 cells using GTX54530 GATA4 antibody compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

IHC-P analysis of mouse pancreas tissue using GTX54530 GATA4 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:500

FACS analysis of NIH/3T3 cells using GTX54530 GATA4 antibody compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature