Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GCN2 antibody (GTX135252) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GCN2 antibody (GTX135252) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.