GDF5 + GDF6 antibody [C3], C-term detects GDF5 protein at cytosol on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
GDF5 + GDF6 antibody [C3], C-term (GTX108415) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX108415 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GDF5 + GDF6 antibody [C3], C-term (GTX108415) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.