Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GDF5(GTX113580) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX113580 diluted at 1:1000

Various whole cell extracts (30 Âμg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GDF5 antibody (GTX113580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

The data was published in the journal PLoS One in 2012. PMID: 23226264

The data was published in the journal PLoS One in 2012. PMID: 23226264

The data was published in the journal Stem Cells Int in 2013. PMID: 24454406