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GFAP antibody [GFAP/2076]

Anti-GFAP antibody [GFAP/2076] used in Flow cytometry (FACS). GTX18062
Anti-GFAP antibody [GFAP/2076] used in IHC (Paraffin sections) (IHC-P). GTX18062

Cat No. GTX18062

Host

Mouse

Clonality

Monoclonal

Clone Name

GFAP/2076

Isotype

IgG1

Application

WB, IHC-P, FACS

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Product Note

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GFAP protein fragment (around aa 101-200) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ALXDRD , GFAP , glial fibrillary acidic protein

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GFAP antibody [GFAP/2076] used in Flow cytometry (FACS). GTX18062

GTX18062 FACS Image

FACS analysis of T98G cells using GTX18062 GFAP antibody [GFAP/2076].
Blue : Primary antibody
Red : Isotype control

Anti-GFAP antibody [GFAP/2076] used in IHC (Paraffin sections) (IHC-P). GTX18062

GTX18062 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX18062 GFAP antibody [GFAP/2076].

REFERENCE

There are currently no references for GFAP antibody [GFAP/2076] (GTX18062). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GFAP antibody [GFAP/2076] (GTX18062). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf