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GFP antibody [9F9.F9]

Anti-GFP antibody [9F9.F9] used in Western Blot (WB). GTX21218
Anti-GFP antibody [9F9.F9] used in Western Blot (WB). GTX21218

Cat No. GTX21218

Host

Mouse

Clonality

Monoclonal

Clone Name

9F9.F9

Isotype

IgG1

Application

WB, ICC/IF, FACS, IP, ELISA, IHC
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:3000-30000
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA 1:10000-30000
IHC 1:1000-5000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium phosphate, 0.15M sodium chloride, pH 7.2, containing 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Jellyfish

Immunogen

The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Purification

IgG fraction

Conjugation

Unconjugated

RRID

AB_371417

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expression and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Research Area

DATA IMAGES

Anti-GFP antibody [9F9.F9] used in Western Blot (WB). GTX21218

GTX21218 WB Image

Western Blot of anti-GFP monoclonal antibody (GTX21218). Lane 1: 64pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates). Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Primary antibody: anti-GFP monoclonal antibody at 1:400 for overnight at 4ºC. Secondary antibody: HRP-conjugated anti-Mouse IgG (GTX26728) was performed at a dilution of 1:20,000 for 1h at 4ºC. Block: TTBS supplemented with 1% BSA for 1 h at 4ºC. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.

Anti-GFP antibody [9F9.F9] used in Western Blot (WB). GTX21218

GTX21218 WB Image

Figure: Western blot of GFP recombinant protein detected with GeneTex monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. GTX21218 detects a 27 kDa band corresponding to the epitope tag GFP. The membrane was probed with the primary antibody diluted to 1.0 μg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDye® 800 conjugated goat anti-Mouse IgG [H&L]. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339