Summary
GFP antibody recognizes the green fluorescent protein (GFP), a fluorophore in widespread use in biomedical research. GFP fluorescence allows real-time study of physiological processes involving fusion proteins or GFP-expressing cells. This antibody also detects other GFP variants, including EGFP and YFP, but not red fluorescent protein (RFP).
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:20000 |
1:1000-1:5000 |
Assay dependent |
Assay dependent |
1:100-1:500 |
1:100-1:500 |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T expressing GFP-tagged protein
Product Note
This antibody reacts with GFP variants including GFP, EGFP, and YFP.
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.17 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Full length GFP recombinant protein
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950371
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.