GeneTex
United States (US)

GFP antibody

GTX113617_40058_IHC-Wm_Z_18121410_683.jpg
GTX113617_WB_GK_18121410_138.jpg
GTX113617_IP_18121410_721.jpg
GTX113617_40555_WB_B_18121410_157.jpg
GTX113617_IFA_18121410_423.jpg
GTX113617_42060_20150618_WB_B_18121410_291.jpg

Cat No. GTX113617

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, ELISA, IHC
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:1000-1:5000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IP 1:100-1:500
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

human GFP-transfected 293T , 293T whole cell extract expressing GFP-tagged protein , *GFP-p53 transfected U2OS

Specificity/Sensitivity

This antibody reacts with GFP variants including GFP, EGFP, and YFP.
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Green Fluorescent Protein, GFP, eGFP, enhanced green fluorescent protein

Research Area

DATA IMAGES
GTX113617_40058_IHC-Wm_Z_18121410_683.jpg

GTX113617 IHC-Wm Image

Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo, using GFP(GTX113617) antibody at 1:200 dilution.

GTX113617_WB_GK_18121410_138.jpg

GTX113617 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113617_IP_18121410_721.jpg

GTX113617 IP Image

GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
The precipitated GFP was detected by GTX113617 diluted at 1:15000.

GTX113617_40555_WB_B_18121410_157.jpg

GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis.
A. 1 µg 293T whole cell extract
B. 1 µg 293T whole cell extract expressing GFP-tagged protein
C. 1 µg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX113617) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113617_IFA_18121410_423.jpg

GTX113617 ICC/IF Image

Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

GTX113617_42060_20150618_WB_B_18121410_291.jpg

GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis. Non-transfected (-) and GFP-transfected (+, ) 293T whole cell extracts (1 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

GFP antibody Cat No. GTX113617

Rating ( Average 4.8 based on 6 users reviews)
Application
Western Blot(WB) ( Average 4.8 based on 4 users reviews)
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Oct-2018
Score :
Application Tested : WB
Sample : BY4741 (budding yeast) expressing GFP(S65T)-tagged Vps21p (small GTPase Rab5 homologue)
Amount used : 5 μg
Blocking : 1% nonfat milk in PBS-0.05% Tween-20, 25°C, 0.5Hr
Primary Antibody : 1:10000, 4°C, 5Hr
Date : Anonymous submitted on 01-Jun-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila eye
Fixation : 4% PFA, 4°C, 0.17Hr
Permeabilization /Antigen retrieval : 0.3% PBST, 10 minHr
Primary Antibody : 1:500, 4°C, overnightHr
Date : Anonymous submitted on 22-Feb-2018
Score :
Application Tested : IHC
Sample : Drosophila Retinal tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, overnightHr
Figure Note : Green: GTX113617 GFP antibody: photoreceptor cell R8, Red: GTX630189 mcherry antibody: medulla
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP recombinant protein transfected 293T cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP-p53 transfected U2OS cells
Amount used : 15μg
Blocking : 5% milk in 0.1%TBST, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : A: U2OS cells transfected with GFP-p53, B: U2OS cells transfected with control plasmid (no GFP-tag)
Date : Anonymous submitted on 12-Apr-2012
Score :
Application Tested : WB
Sample : Protoplast from Rosette leaves
Amount used : 30μg
Blocking : 5% milk in PBST, 4°C, 16Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169