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GFP antibody

Orthogonal Overexpression
Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617

Cat. No. GTX113617

Reference 160
22

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, Dot, ELISA, IHC
Package
100 μl ($399),
25 μl ($169)
See all GFP products
PRODUCT

Summary

GFP antibody recognizes the green fluorescent protein (GFP), a fluorophore in widespread use in biomedical research. GFP fluorescence allows real-time study of physiological processes involving fusion proteins or GFP-expressing cells. This antibody also detects other GFP variants, including EGFP and YFP, but not red fluorescent protein (RFP).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:1000-1:5000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IP 1:100-1:500
Dot Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T expressing GFP-tagged protein

Product Note

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950371

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Green Fluorescent Protein , GFP , eGFP , enhanced green fluorescent protein

Research Area

DATA IMAGES

Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617

GTX113617 IHC-Wm Image

Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo (fixed with ice-cold acetone), using GFP(GTX113617) antibody at 1:200 dilution.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617

GTX113617 IP Image

GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
The precipitated GFP was detected by GTX113617 diluted at 1:15000.

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617

GTX113617 ICC/IF Image

Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

GFP antibody detects GFP protein by Western blot analysis. Non-transfected (-) and GFP-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) diluted at 1:10000.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis.
A. 1 μg 293T whole cell extract
B. 1 μg 293T whole cell extract expressing GFP-tagged protein
C. 1 μg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX113617) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Rimmer N et al. STAR Protoc 2023; 4 (2) : 102305 Generation of endogenously tagged E-cadherin cells using gene editing via non-homologous end joining.
Zhao B et al. Cell Oncol (Dordr) 2023; 46 (3) : 717-733 USP13 promotes breast cancer metastasis through FBXL14-induced Twist1 ubiquitination.
Wu T et al. Elife 2023; 12 Tau polarizes an aging transcriptional signature to excitatory neurons and glia.
M Huang et al. Cell Rep 2023; 42 (6) : 112667 LRP12 is an endogenous transmembrane inactivator of α4 integrins
Y Li et al. Cell Death Dis 2023; KLF12 promotes the proliferation of breast cancer cells by reducing the transcription of p21 in a p53-dependent and p53-independent manner
Application : WB, IP
Show More +
Submit a Reference

REVIEW

GFP antibody Cat. No. GTX113617

Rating
( Average 5 based on 22 users reviews)
Application
Western Blot(WB)
( Average 4.9 based on 17 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 3 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB,IP
Figure Note : The data was peer reviewed and published in the 2020 in Front Mol Neurosci. PMID: 32587503
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 08-Mar-2022
Score :
Application Tested : WB
Sample : Transgenic mouse treated with TAM
Amount used : 20μg
Blocking : 5% gelatin in T-TBS, 20°C, 0.5Hr
Primary Antibody : 1:1500, 4°C, 16Hr
Figure Note : Intestinal specific GFP expression was examined in transgenic mouse treated with TAM.
Anti-GFP antibody used in IHC (Paraffin sections) (IHC-P). GTX113617
Date : Anonymous submitted on 31-Aug-2021
Score :
Application Tested : IHC-P
Sample : mFLE-EGFP mouse testis, 2-dpp, paraffin-section 5μm
Blocking : 5% donkey-serum in TNB, 0.3% Triton X-100, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : The 2-dpp testis tissue section of mFLE-EGFP transgenic mouse which present fetal Leydig cell (fLC) was stained by GFP antibody and HSD-3b1 antibody. GFP only expressed in fLC.
Anti-GFP antibody used in IHC (Frozen sections) (IHC-Fr). GTX113617
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018.PMID: 30310071
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2018.PMID: 29361943
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IP,WB
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018.PMID: 29752474
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018.PMID: 29752474
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018.PMID: 29752474
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Figure Note : The data was peer reviewed and published in the journal J Virol in 2016. PMID: 27489281
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Adv Sci (Weinh) in 2020. PMID: 32195080
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Commun Biol in 2019. PMID: 31754649
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Commun Biol in 2019. PMID: 31754649
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Commun Biol in 2019. PMID: 31754649
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019. PMID: 31311575
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019. PMID: 31311575
Anti-GFP antibody used in IHC (Paraffin sections) (IHC-P). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2014. PMID: 24448489
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Oct-2018
Score :
Application Tested : WB
Sample : BY4741 (budding yeast) expressing GFP(S65T)-tagged Vps21p (small GTPase Rab5 homologue)
Amount used : 5 μg
Blocking : 1% nonfat milk in PBS-0.05% Tween-20, 25°C, 0.5Hr
Primary Antibody : 1:10000, 4°C, 5Hr
Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617
Date : Anonymous submitted on 01-Jun-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila eye
Fixation : 4% PFA, 4°C, 0.17Hr
Permeabilization /Antigen retrieval : 0.3% PBST, 10 minHr
Primary Antibody : 1:500, 4°C, 16Hr
Anti-GFP antibody used in Immunohistochemistry (IHC). GTX113617
Date : Anonymous submitted on 22-Feb-2018
Score :
Application Tested : IHC
Sample : Drosophila Retinal tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Green: GTX113617 GFP antibody: photoreceptor cell R8, Red: GTX630189 mcherry antibody: medulla
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP recombinant protein transfected 293T cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP-p53 transfected U2OS cells
Amount used : 15μg
Blocking : 5% milk in 0.1%TBST, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : A: U2OS cells transfected with GFP-p53, B: U2OS cells transfected with control plasmid (no GFP-tag)
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 12-Apr-2012
Score :
Application Tested : WB
Sample : Protoplast from Rosette leaves
Amount used : 30μg
Blocking : 5% milk in PBST, 4°C, 16Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Show More +
Submit a Review
Application Reference
Rimmer N et al. STAR Protoc 2023; 4 (2):102305 Generation of endogenously tagged E-cadherin cells using gene editing via non-homologous end joining.
Zhao B et al. Cell Oncol (Dordr) 2023; 46 (3):717-733 USP13 promotes breast cancer metastasis through FBXL14-induced Twist1 ubiquitination.
Wu T et al. Elife 2023; 12 Tau polarizes an aging transcriptional signature to excitatory neurons and glia.
M Huang et al. Cell Rep 2023; 42 (6):112667 LRP12 is an endogenous transmembrane inactivator of α4 integrins
Y Li et al. Cell Death Dis 2023; KLF12 promotes the proliferation of breast cancer cells by reducing the transcription of p21 in a p53-dependent and p53-independent manner
Application : WB, IP
Gu NX et al. Development 2023; 150 (4) Frizzled 7 modulates goblet and Paneth cell fate, and maintains homeostasis in mouse intestine.
Application : IHC-P
PL Hsu et al. Sci Adv 2023; 9 (15):eade3422 Targeting BRD3 eradicates nuclear TYRO3-induced colorectal cancer metastasis
Application : WB, IHC-P
Adriaenssens E et al. Nat Cell Biol 2023; 25 (3):467-480 Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space.
Application : WB
Li M et al. Int J Mol Sci 2023; 24 (1) Novel Missense Variants in PAX8 and NKX2-1 Cause Congenital Hypothyroidism.
Application : WB, ICC/IF
Wang HW et al. J Biomed Sci 2023; 30 (1):12 CLEC5A mediates Zika virus-induced testicular damage.
Blum N et al. Dis Model Mech 2023; 16 (2) Localized heterochrony integrates overgrowth potential of oncogenic clones.
Chao TY et al. Microbiol Spectr 2023; 11 (1):e0436922 Subcellular Distribution of BALF2 and the Role of Rab1 in the Formation of Epstein-Barr Virus Cytoplasmic Assembly Compartment and Virion Release.
Application : WB, IP
Uszczynska-Ratajczak B et al. Life Sci Alliance 2023; 6 (1) Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish.
Deng N et al. Proc Natl Acad Sci U S A 2022; 119 (32):e2204779119 Chemical interference with DSIF complex formation lowers synthesis of mutant huntingtin gene products and curtails mutant phenotypes.
Application : WB
Petrosino S et al. Int J Mol Sci 2022; 24 (1) HMGA1 Regulates the Expression of Replication-Dependent Histone Genes and Cell-Cycle in Breast Cancer Cells.
Yamada S et al. PLoS Genet 2022; 18 (10):e1010438 Inka2, a novel Pak4 inhibitor, regulates actin dynamics in neuronal development.
Chen JC et al. Stem Cell Res Ther 2022; 13 (1):498 Enteric neurosphere cells injected into rectal submucosa might migrate caudorostrally to reconstitute enteric ganglia along the entire length of postnatal colon.
Lin MJ et al. Front Cell Dev Biol 2022; 10:907863 Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line.
Yin P et al. Autophagy 2022; 18 (8):1955-1968 SQSTM1-mediated clearance of cytoplasmic mutant TARDBP/TDP-43 in the monkey brain.
Application : WB, ICC/IF, IP
Su W et al. Nat Commun 2022; 13 (1):6577 Phosphorylation of 17β-hydroxysteroid dehydrogenase 13 at serine 33 attenuates nonalcoholic fatty liver disease in mice.
Application : WB, ICC/IF, IP
Larasati YA et al. Sci Adv 2022; 8 (40):eabn9350 Restoration of the GTPase activity and cellular interactions of Gαo mutants by Zn2+ in GNAO1 encephalopathy models.
Application : WB
Xu Z et al. Cell Death Discov 2022; 8 (1):474 Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer.
Shu-Jung Chang et al. Elife 2022; 11 Typhoid toxin sorting and exocytic transport from Salmonella Typhi-infected cells.
Application : WB
Guofu Zhu et al. Br J Pharmacol 2022; 179 (8):1716-1731 A novel peptide inhibitor of Dll4-Notch1 signalling and its pro-angiogenic functions.
Application : IP
Gonzalo P Solis et al. Nat Commun 2022; 13 (1):2072 Local and substrate-specific S-palmitoylation determines subcellular localization of Gαo.
Application : WB
Mikkel ? N?rg?rd et al. Sci Rep 2022; 12 (1):496 A new transgene mouse model using an extravesicular EGFP tag enables affinity isolation of cell-specific extracellular vesicles.
Application : WB
Miete C et al. Nat Commun 2022; 13 (1):674 Gαi2-induced conductin/axin2 condensates inhibit Wnt/β-catenin signaling and suppress cancer growth.
Application : WB
Bai F et al. Theranostics 2022; 12 (2):720-733 Loss of function of GATA3 induces basal-like mammary tumors.
Rochford I et al. Am J Physiol Lung Cell Mol Physiol 2021; 321 (4):L686-L702 Evidence for reprogramming of monocytes into reparative alveolar macrophages in vivo by targeting PDE4b.
Seiya Yamada et al. iScience 2021; 24 (12):103484 Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development.
Application : WB
Ke Zhang et al. Sci Adv 2021; 7 (6) Nsp1 protein of SARS-CoV-2 disrupts the mRNA export machinery to inhibit host gene expression.
Application : ICC/IF
Han-Yu Wang et al. Cells 2021; 10 (2) Protein Kinase A-Mediated Septin7 Phosphorylation Disrupts Septin Filaments and Ciliogenesis.
Application : WB, ICC/IF
Jenn-Haung Lai et al. iScience 2021; 24 (6):102498 Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways.
Application : WB
Hsin-Hsin Peng et al. Aging (Albany NY) 2021; 13 (10):13474-13495 Ganoderma lucidum stimulates autophagy-dependent longevity pathways in Caenorhabditis elegans and human cells.
Application : WB
Jie-Ning Li et al. Front Cell Dev Biol 2021; 9:671244 E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity.
Application : WB
Chao-Jung Chen et al. Front Pharmacol 2021; 12:720821 Effect of Xanthium Strumarium on HIV-1 5'-LTR Transcriptional Activity and Viral Reactivation in Latently Infected Cells.
Application : WB
Aman S et al. Cell Death Discov 2021; 7 (1):351 DACH1 inhibits breast cancer cell invasion and metastasis by down-regulating the transcription of matrix metalloproteinase 9.
Application : WB, IP
Xiahui Li et al. NPJ Precis Oncol. 2021; 5 (1):20 Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1.
Application : WB
Solis GP et al. Cells 2021; 10 (10) Pediatric Encephalopathy: Clinical, Biochemical and Cellular Insights into the Role of Gln52 of GNAO1 and GNAI1 for the Dominant Disease.
Kramer J et al. PLoS One 2021; 16 (10):e0258872 Dpp/TGFβ-superfamily play a dual conserved role in mediating the damage response in the retina.
Yang Y et al. Cell Death Discov 2021; 7 (1):328 FBXL10 promotes EMT and metastasis of breast cancer cells via regulating the acetylation and transcriptional activity of SNAI1.
Chang YC et al. Nat Commun 2021; 12 (1):4258 VCP maintains nuclear size by regulating the DNA damage-associated MDC1-p53-autophagy axis in Drosophila.
Application : WB
Hsieh YC et al. Cell Mol Gastroenterol Hepatol 2021; 12 (3):813-838 (Pro)renin Receptor Knockdown Attenuates Liver Fibrosis Through Inactivation of ERK/TGF-β1/SMAD3 Pathway.
Application : IHC
Miki T et al. Nat Commun 2021; 12 (1):3412 Intracellular artificial supramolecules based on de novo designed Y15 peptides.
Application : WB
Ou M et al. Oncol Rep 2021; 46 (1) MDM2 induces EMT via the B?Raf signaling pathway through 14?3?3.
Application : WB
Li B et al. Cancer Cell Int 2021; 21 (1):38 TIMELESS inhibits breast cancer cell invasion and metastasis by down-regulating the expression of MMP9.
Application : WB
Fan Z et al. Antimicrob Agents Chemother 2021; 65 (2) Pseudomonas aeruginosa Polynucleotide Phosphorylase Controls Tolerance to Aminoglycoside Antibiotics by Regulating the MexXY Multidrug Efflux Pump.
Application : WB
Yin D et al. Nat Biotechnol 2021; Targeting herpes simplex virus with CRISPR-Cas9 cures herpetic stromal keratitis in mice.
Application : IHC-Fr
Huang YL et al. Elife 2020; 9 Collagen-rich omentum is a premetastatic niche for integrin α2-mediated peritoneal metastasis.
Chang YJ et al. Cancers (Basel) 2020; 12 (10) Mitochondrial ROS1 Increases Mitochondrial Fission and Respiration in Oral Squamous Cancer Carcinoma.
Fuchigami T et al. J Appl Oral Sci 2020; 28:e20190558 Ameloblastoma cell lines derived from different subtypes demonstrate distinct developmental patterns in a novel animal experimental model.
Young TJ et al. PLoS Genet 2020; 16 (12):e1009226 CAF-1 and Rtt101p function within the replication-coupled chromatin assembly network to promote H4 K16ac, preventing ectopic silencing.
Application : WB
Kumar R et al. Adv Sci (Weinh) 2020; 7 (6):1902372 Fibroblast Growth Factor 2-Mediated Regulation of Neuronal Exosome Release Depends on VAMP3/Cellubrevin in Hippocampal Neurons.
Hao P et al. Life Sci 2020; 262:118492 Lipocalin2 promotes cell proliferation and migration in ovarian cancer through activation of the ERK/GSK3β/β-catenin signaling pathway.
Application : WB
Dai YC et al. J Virol 2020; 94 (3) The Novel Nuclear Targeting and BFRF1-Interacting Domains of BFLF2 Are Essential for Efficient Epstein-Barr Virus Virion Release.
Meng J et al. Small 2020; 16 (31):e2001890 Synthetic CXCR4 Antagonistic Peptide Assembling with Nanoscaled Micelles Combat Acute Myeloid Leukemia.
Wu AY et al. Adv Sci (Weinh) 2020; 7 (19):2001467 Multiresolution Imaging Using Bioluminescence Resonance Energy Transfer Identifies Distinct Biodistribution Profiles of Extracellular Vesicles and Exomeres with Redirected Tropism.
Application : Dot
Seguin A et al. J Biol Chem 2020; 295 (32):11002-11020 The mitochondrial metal transporters mitoferrin1 and mitoferrin2 are required for liver regeneration and cell proliferation in mice.
Tsai HY et al. Dis Model Mech 2020; 13 (8) Loss of the Drosophila branched-chain α-ketoacid dehydrogenase complex results in neuronal dysfunction.
Application : WB
Bernadzki KM et al. Front Mol Neurosci 2020; 13:104 Arhgef5 Binds α-Dystrobrevin 1 and Regulates Neuromuscular Junction Integrity.
Application : IP
Fukushima Y et al. EMBO J 2020; 39 (12):e102930 RhoJ integrates attractive and repulsive cues in directional migration of endothelial cells.
Application : WB, IHC-Wm
Wang IH et al. Cancers (Basel) 2020; 12 (5) Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression.
Application : WB
Seiya Yamada et al. iScience 2020; 23 (5):101058 Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.
Application : IHC-Fr
Anthony Yan-Tang Wu et al. bioRxiv 2020; (Epub) Redirected tropisms of extracellular vesicles and exomeres yield distinct biodistribution profiles.
Pei YF et al. Am J Pathol 2020; 190 (6):1343-1354 The Crosstalk between lncRNA-SNHG7/miRNA-181/cbx7 Modulates Malignant Character in Lung Adenocarcinoma.
Application : WB
Chen X et al. Hum Gene Ther 2020; 31 (7-8):440-447 Different Serotypes of Adeno-Associated Virus Vector- and Lentivirus-Mediated Tropism in Choroid Plexus by Intracerebroventricular Delivery.
Application : ICC/IF
Chen CH et al. Biochem Biophys Res Commun 2020; 524 (4):1003-1009 Dicoumarol suppresses HMGA2-mediated oncogenic capacities and inhibits cell proliferation by inducing apoptosis in colon cancer.
Application : WB
Iwaizumi M et al. Fish Physiol Biochem 2020; (Epub) Plasmid delivery by electroporation into fish skeletal muscle for recombinant protein secretion and uptake by oocytes.
Application : IHC-P
Rohit Kumar et al. Advanced Science 2020; (Epub) Fibroblast Growth Factor 2?Mediated Regulation of Neuronal Exosome Release Depends on VAMP3/Cellubrevin in Hippocampal Neurons.
Akiyama H et al. Cell Tissue Res 2020; (Epub) Control of cell migration by the novel protein phosphatase-2A interacting protein inka2.
Application : WB
Sarkar A et al. Haematologica 2020; (Epub) Ataxia telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma.
Application : WB
Chen PH et al. FASEB J 2020; 34 (1):1107-1121 Nucleolar control by a non-apoptotic p53-caspases-deubiquitinylase axis promotes resistance to bacterial infection.
Application : WB
Nagano M et al. Commun Biol 2019; 2 (1):419 Rab5-mediated endosome formation is regulated at the trans-Golgi network.
Application : WB
Cassidy KB et al. FEBS J 2019; (Epub) Direct regulation of Chk1 protein stability by E3 ubiquitin ligase HUWE1.
Application : WB
Ke YT et al. G3 (Bethesda) 2019; 9 (6):2007-2016 Generation of Inducible Gene-Switched GAL4 Expressed in the Drosophila Female Germline Stem Cell Niche.
Nagano M et al. Commun Biol 2019; 2:419 Rab5-mediated endosome formation is regulated at the trans-Golgi network.
Application : WB
Ngo AM et al. Elife 2019; 8 The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
Application : WB
Zanin R et al. J Exp Clin Cancer Res 2019; 38 (1):313 HMGA1 promotes breast cancer angiogenesis supporting the stability, nuclear localization and transcriptional activity of FOXM1.
Application : IP
Shull TE et al. Environ Sci Technol 2019; 53 (16):9522-9532 Anatase TiO2 Nanoparticles Induce Autophagy and Chloroplast Degradation in Thale Cress (Arabidopsis thaliana).
Application : WB
Chang CH et al. Cell Rep 2019; 28 (5):1195-1205.e3 The Ventral Hippocampus Controls Stress-Provoked Impulsive Aggression through the Ventromedial Hypothalamus in Post-Weaning Social Isolation Mice.
Application : IHC-Fr
Lai TC et al. Sci Rep 2019; 9 (1):5304 Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism.
Application : WB
Fern?ndez MR et al. Methods Mol Biol 2019; 1958:237-261 Formation of Cross-Beta Supersecondary Structure by Soft-Amyloid Cores: Strategies for Their Prediction and Characterization.
Li H et al. Int J Med Sci 2019; 16 (1):106-114 MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation.
Kozareva DA et al. Behav Brain Res 2019; 360:36-50 TLX knockdown in the dorsal dentate gyrus of juvenile rats differentially affects adolescent and adult behaviour.
Application : IHC-Fr
Tanaka J et al. Nat Commun 2018; 9 (1):4216 Generation of orthotopically functional salivary gland from embryonic stem cells.
Yang HL et al. J Cell Physiol 2018; (Epub) Induction of autophagic cell death in human ovarian carcinoma cells by Antrodia salmonea through increased reactive oxygen species generation.
Ford MJ et al. Dev Cell 2018; 47 (4):509-523.e5 A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.
Tsai TL et al. J Virol 2018; (Epub) Interplay between the poly(A) tail, poly(A)-binding protein and coronavirus nucleocapsid protein regulates gene expression of the coronavirus and host cell.
Application : WB
Hu CM et al. FASEB J 2018;:fj201800856R Thymidylate kinase is critical for DNA repair via ATM-dependent Tip60 complex formation.
Application : ICC/IF
Cheng C et al. Hum Mol Genet 2018; (Epub) The long noncoding RNA NEAT1 is elevated in polyglutamine repeat expansion diseases and protects from disease-gene dependent toxicities.
Application : WB
Lai CY et al. Oncogene 2018; (Epub) Identification of UAP1L1 as a critical factor for protein O-GlcNAcylation and cell proliferation in human hepatoma cells.
Application : ICC/IF
Koba S et al. J Physiol 2018; (Epub) Sympathoexcitation by hypothalamic paraventricular nucleus neurons projecting to the rostral ventrolateral medulla.
Application : ICC/IF
Meriin AB et al. Proc Natl Acad Sci U S A 2018; (Epub) Hsp70-Bag3 complex is a hub for proteotoxicity-induced signaling that controls protein aggregation.
Schillemans M et al. Arterioscler Thromb Vasc Biol 2018; (Epub) The Weibel-Palade Body Localized SNARE (Soluble NSF Attachment Protein Receptor) Syntaxin-3 Modulates Von Willebrand Factor Secretion From Endothelial Cells.
Application : WB
Shukla S et al. Methods Mol Biol 2018; 1776:61-84 In Planta Production of Fluorescent Filamentous Plant Virus-Based Nanoparticles.
Bondar VV et al. Hum Mol Genet 2018; (Epub) PAK1 regulates ATXN1 levels providing an opportunity to modify its toxicity in Spinocerebellar ataxia type 1.
Application : WB
Xu Z et al. Cell Death Dis 2018; 9 (5):559 Checkpoint suppressor 1 suppresses transcriptional activity of ERα and breast cancer cell proliferation via deacetylase SIRT1.
Dodson M et al. Mol Cell Biol 2018; (Epub) Increased O-GlcNAcylation of SNAP29 drives arsenic-induced autophagic dysfunction.
Borga C et al. bioRxiv 2018; (Epub) Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis.
Application : IHC-P
Chou YT et al. J Biomed Sci 2018; 25 (1):6 Identification of as an isoform-specific inhibitor of HEME OXYGENASE-1/cytochrome P450 reductase interaction with anti-tumor activity.
Application : WB
Zhu Y et al. J Cell Biochem 2018; (Epub) Autophagy inhibition promotes phagocytosis of macrophage and protects mice from methicillin-resistant staphylococcus aureus pneumonia.
Application : ICC/IF
Xie WR et al. J Neurosci 2017; 37 (36):8583-8594 An Atoh1-S193A Phospho-Mutant Allele Causes Hearing Deficits and Motor Impairment.
Lu JW et al. Blood Cancer J 2017; 7 (8):e593 Overexpression of SOX4 correlates with poor prognosis of acute myeloid leukemia and is leukemogenic in zebrafish.
Vega-Cabrera LA et al. PLoS One 2017; 12 (2):e0172737 Analysis of Spo0M function in Bacillus subtilis.
Gao Z et al. Development 2017; (Epub) PDGFRα / PDGFRβ signaling balance modulates progenitor cell differentiation into white and beige adipocytes.
Tzeng YW et al. Int J Biochem Cell Biol 2017; 94:22-30 LMO7 exerts an effect on mitosis progression and the spindle assembly checkpoint.
Application : WB, IP
van Ijzendoorn DGP et al. J Biol Chem 2017; (Epub) Functional Analyses of a Human Vascular Tumor FOS Variant Identify a Novel Degradation Mechanism and a link to Tumorigenesis.
Suboti? A et al. G3 (Bethesda) 2017; 7 (10):3509-3520 A Bimolecular Fluorescence Complementation Tool for Identification of Protein-Protein Interactions in Candida albicans.
Application : WB
Chang CT et al. Food Chem Toxicol 2017; 103:43117 Inhibition of ROS production, autophagy or apoptosis signaling reversed the anticancer properties of Antrodia salmonea in triple-negative breast cancer (MDA-MB-231) cells.
Application : ICC/IF
Solis GP et al. Cell 2017; (Epub) Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.
Application : WB
Abiria SA et al. Proc Natl Acad Sci U S A 2017; TRPM7 senses oxidative stress to release Zn2+ from unique intracellular vesicles.
Application : IP
Tillotson J et al. Chem Res Toxicol 2017; 30 (7):1508-1514 Arsenic Compromises Both p97 and Proteasome Functions.
Application : WB
Wu SC et al. Nat Commun 2017; 8 (1):24 Intestinal microbial dysbiosis aggravates the progression of Alzheimer's disease in Drosophila.
Chang CT et al. Arch Toxicol 2017; (Epub) Chalcone flavokawain B induces autophagic-cell death via reactive oxygen species-mediated signaling pathways in human gastric carcinoma and suppresses tumor growth in nude mice.
Application : WB
Harder B et al. Mol Carcinog 2016; (Epub) Brusatol overcomes chemoresistance through inhibition of protein translation.
Nyman JS et al. Bone 2016; 91:81-91 combined treatment with a transforming growth factor beta inhibitor (1d11) and * improves bone architecture in a mouse model of myeloma-induced bone disease.
Application : IHC-P
Koizumi H et al. Dev Neurobiol 2016; DCLK1 phosphorylates the microtubule-associated protein MAP7D1 to promote axon elongation in cortical neurons.
Wong MT et al. J Virol 2016; Human Choline Kinase-α Promotes Hepatitis C Virus RNA Replication through Modulating Membranous Viral Replication Complex Formation.
Application : WB
Lai CW et al. Mol Imaging Biol 2016; (Epub) Using Dual Fluorescence Reporting Genes to Establish an In Vivo Imaging Model of Orthotopic Lung Adenocarcinoma in Mice.
Application : WB, IHC-P
Lai CW et al. Sci Rep 2016; 6:24023 Sexually Dimorphic Expression of eGFP Transgene in the Akr1A1 Locus of Mouse Liver Regulated by Sex Hormone-Related Epigenetic Remodeling.
Application : WB, IHC-P
Xu Q et al. BMC Evol Biol 2016; 16 (1):11 Analysis of the erythropoietin of a Tibetan Plateau schizothoracine fish (Gymnocypris dobula) reveals enhanced cytoprotection function in hypoxic environments.
Wu HH et al. J Exp Med 2015; 212 (3):333-49 Targeting IL-17B-IL-17RB signaling with an anti-IL-17RB antibody blocks pancreatic cancer metastasis by silencing multiple chemokines.
Yu H et al. Biosci Rep 2015; 35 (3) Autonomous and non-autonomous roles of DNase-II during cell death in C. elegans embryos.
Application : IHC-Wm
Steinke KV et al. J Biol Chem 2015; 290 (28):17390-400 Human CLC-K Channels Require Palmitoylation of Their Accessory Subunit Barttin to Be Functional.
Application : IP
Ross E et al. PLoS One 2015; 10 (5):e0128013 AMP-Activated Protein Kinase Regulates the Cell Surface Proteome and Integrin Membrane Traffic.
Application : WB
Yang FM et al. Biochem J 2015; 471 (2):199-209 A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding.
Application : IP
Dickmeis C et al. Front Bioeng Biotechnol 2015; 3:189 Production of Hybrid Chimeric PVX Particles Using a Combination of TMV and PVX-Based Expression Vectors.
Application : WB
Chen PJ et al. Nucleic Acids Res 2015; 43 (19):9393-404 NPGPx modulates CPEB2-controlled HIF-1α RNA translation in response to oxidative stress.
Li Q et al. J Biol Chem 2015; 290 (35):21553-67 Akt Phosphorylates Wnt Coactivator and Chromatin Effector Pygo2 at Serine 48 to Antagonize Its Ubiquitin/Proteasome-mediated Degradation.
Wu JM et al. Circ Res 2015; 116 (4):633-41 Circulating cells contribute to cardiomyocyte regeneration after injury.
Application : IHC-P
Lee WY et al. Oncotarget 2015; (Epub) repositioning antipsychotic * for treating colorectal cancer by inhibiting sirtuin 1.
Application : WB
Chen CJ et al. J Biol Chem 2015; 290 (10):6010-21 SH2B1 and IRSp53 Proteins Promote the Formation of Dendrites and Dendritic Branches.
Application : WB, ICC/IF
Wu HC et al. Biotechnol Prog 2015; Production and characterization of active recombinant interleukin-12/eGFP fusion protein in stably-transfected DF1 chicken cells.
Application : ELISA
Auer TO et al. Genome Res 2014; 24 (1):142-53 Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair.
Application : IHC-Wm
Hsueh YC et al. EMBO Mol Med 2014; 6 (4):496-503 Prostaglandin E- promotes post-infarction cardiomyocyte replenishment by endogenous stem cells.
Application : IHC-P
Willemen HL et al. J Pain 2014; 15 (5):496-506 Monocytes/Macrophages control resolution of transient inflammatory pain.
Application : IHC-Fr
Hong JB et al. PLoS One 2014; 9 (2):e89396 A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells.
Application : WB
Pope-Varsalona H et al. Nucleic Acids Res 2014; 42 (21):13096-109 Polymerase η suppresses telomere defects induced by DNA damaging agents.
Application : ICC/IF
Huang IH et al. J Cell Sci. 2014; 127 (Pt 19):4186-200 GEF-H1 controls focal adhesion signaling that regulates mesenchymal stem cell lineage commitment.
Application : WB
Yu Z et al. Cell Signal 2014; 26 (7):1560-1566 Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1α in HepG2 cells.
Application : WB
S Shukla et al. Biomater Sci 2014; Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models
Hsu CY et al. PLoS Pathog 2014; 10 (2):e1003974 The Epstein-Barr Virus-Encoded MicroRNA MiR-BART9 Promotes Tumor Metastasis by Targeting E-Cadherin in Nasopharyngeal Carcinoma.
Application : IHC
Chang YJ et al. Mol Cell Biol 2014; 34 (6):1003-19 SH2B1β Interacts with STAT3 and Enhances Fibroblast Growth Factor 1-Induced Gene Expression during Neuronal Differentiation.
Application : WB, IP
Mace PD et al. Nat Commun 2013; 4:1681 Structure of ERK2 bound to PEA-15 reveals a mechanism for * of activated MAPK.
Application : WB
Reetz J et al. PLoS One 2013; 8 (6):e67091 Development of Adenoviral Delivery Systems to Target Hepatic Stellate Cells In Vivo.
Application : IHC-P
Molleken K et al. PLoS Pathog 2013; 9 (4):e1003325 The Chlamydia pneumoniae invasin protein Pmp21 recruits the EGF receptor for host cell entry.
Wirth A et al. Biochem J 2013; 456 (3):311-22 Dual lipidation of the brain-specific Cdc42 isoform regulates its functional properties.
Barak LS et al. Biochemistry 2013; 52 (32):5403-14 Triphenylmethane dye activation of beta-arrestin.
Chang RY et al. Vet Microbiol 2013; 166 (43102):43425 japanese encephalitis virus non-coding rna inhibits activation of * by blocking nuclear translocation of * regulatory factor 3.
Application : IP
Park J et al. Nature 2013; 498 (7454):325-31 RAS-MAPK-MSK1 pathway modulates ataxin 1 protein levels and toxicity in SCA1.
Application : WB
Lin LT et al. J Biomed Opt 2013; 18 (12):126013 mPlum-IFP 1.4 fluorescent fusion protein may display F?rster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging.
Application : WB
Wang H et al. J Clin Invest 2013; 123 (12):5023-34 Balancing GRK2 and EPAC1 levels prevents and relieves chronic pain.
Application : IHC-Fr
He G et al. Cell 2013; 155 (2):384-96 Identification of liver cancer progenitors whose malignant progression depends on autocrine IL-6 signaling.
Application : IHC-P
Chiang YJ et al. PLoS One 2013; 8 (4):e61761 CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration.
Application : WB
Tsou TC et al. Toxicol Appl Pharmacol 2013; 273 (3):492-9 Crucial role of Toll-like receptors in the zinc/nickel-induced inflammatory response in vascular endothelial cells.
Application : WB
Hsu WB et al. J Virol 2013; 87 (1):110-23 Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation.
Application : WB
Wei PC et al. Mol Cell 2012; 48 (5):747-59 Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease.
Application : WB
Lai SL et al. Development 2012; 139 (23):4439-48 Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish.
Hsiao WC et al. Mol Pharm 2012; 9 (5):1396-408 Vitamin D3-inducible mesenchymal stem cell-based delivery of conditionally replicating adenoviruses effectively targets renal cell carcinoma and inhibits tumor growth.
Application : IHC
Kwanchanok S et al. Biosci Biotechnol Biochem 2011; 75 (10):37073 In vitro fluorescent analysis of preprotein import into chloroplasts.
Application : WB
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