Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:5000-1:20000 |
1:1000-1:5000 |
Assay dependent |
Assay dependent |
1:100-1:500 |
1:100-1:500 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
human GFP-transfected 293T , 293T whole cell extract expressing GFP-tagged protein , *GFP-p53 transfected U2OS
Specificity/Sensitivity
This antibody reacts with GFP variants including GFP, EGFP, and YFP.
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Immunogen
Full length GFP recombinant protein
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.