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GFP antibody

Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617

Cat No. GTX113617

Reference 77
6
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, ELISA, IHC
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
See all GFP products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:1000-1:5000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IP 1:100-1:500
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

human GFP-transfected 293T , 293T whole cell extract expressing GFP-tagged protein , *GFP-p53 transfected U2OS

Specificity/Sensitivity

This antibody reacts with GFP variants including GFP, EGFP, and YFP.
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Green Fluorescent Protein, GFP, eGFP, enhanced green fluorescent protein

Research Area

DATA IMAGES
Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617

GTX113617 IHC-Wm Image

Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo, using GFP(GTX113617) antibody at 1:200 dilution.
Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617

GTX113617 IP Image

GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
The precipitated GFP was detected by GTX113617 diluted at 1:15000.
Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis.
A. 1 µg 293T whole cell extract
B. 1 µg 293T whole cell extract expressing GFP-tagged protein
C. 1 µg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX113617) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617

GTX113617 ICC/IF Image

Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.
Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis. Non-transfected (-) and GFP-transfected (+, ) 293T whole cell extracts (1 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE

Application Reference

Lai TC et al. Sci Rep 2019; 9 (1) : 5304 Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism.
Application : WB
Reactivity : Human
Fern?ndez MR et al. Methods Mol Biol 2019; 1958 : 237-261 Formation of Cross-Beta Supersecondary Structure by Soft-Amyloid Cores: Strategies for Their Prediction and Characterization.
Li H et al. Int J Med Sci 2019; 16 (1) : 106-114 MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation.
Kozareva DA et al. Behav Brain Res 2019; 360 : 36-50 TLX knockdown in the dorsal dentate gyrus of juvenile rats differentially affects adolescent and adult behaviour.
Application : IHC-Fr
Yang HL et al. J Cell Physiol 2018; (Epub) Induction of autophagic cell death in human ovarian carcinoma cells by Antrodia salmonea through increased reactive oxygen species generation.
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REVIEW

GFP antibody Cat No. GTX113617

Rating
( Average 4.8 based on 6 users reviews)
Application
Western Blot(WB)
( Average 4.8 based on 4 users reviews)
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Oct-2018
Score :
Application Tested : WB
Sample : BY4741 (budding yeast) expressing GFP(S65T)-tagged Vps21p (small GTPase Rab5 homologue)
Amount used : 5 μg
Blocking : 1% nonfat milk in PBS-0.05% Tween-20, 25°C, 0.5Hr
Primary Antibody : 1:10000, 4°C, 5Hr
Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617
Date : Anonymous submitted on 01-Jun-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila eye
Fixation : 4% PFA, 4°C, 0.17Hr
Permeabilization /Antigen retrieval : 0.3% PBST, 10 minHr
Primary Antibody : 1:500, 4°C, overnightHr
Anti-GFP antibody used in Immunohistochemistry (IHC). GTX113617
Date : Anonymous submitted on 22-Feb-2018
Score :
Application Tested : IHC
Sample : Drosophila Retinal tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, overnightHr
Figure Note : Green: GTX113617 GFP antibody: photoreceptor cell R8, Red: GTX630189 mcherry antibody: medulla
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP recombinant protein transfected 293T cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP-p53 transfected U2OS cells
Amount used : 15μg
Blocking : 5% milk in 0.1%TBST, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : A: U2OS cells transfected with GFP-p53, B: U2OS cells transfected with control plasmid (no GFP-tag)
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 12-Apr-2012
Score :
Application Tested : WB
Sample : Protoplast from Rosette leaves
Amount used : 30μg
Blocking : 5% milk in PBST, 4°C, 16Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Show More +
Submit a Review
Application Reference
Lai TC et al. Sci Rep 2019; 9 (1):5304 Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism.
Application : WB
Reactivity : Human
Fern?ndez MR et al. Methods Mol Biol 2019; 1958:237-261 Formation of Cross-Beta Supersecondary Structure by Soft-Amyloid Cores: Strategies for Their Prediction and Characterization.
Li H et al. Int J Med Sci 2019; 16 (1):106-114 MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation.
Kozareva DA et al. Behav Brain Res 2019; 360:36-50 TLX knockdown in the dorsal dentate gyrus of juvenile rats differentially affects adolescent and adult behaviour.
Application : IHC-Fr
Yang HL et al. J Cell Physiol 2018; (Epub) Induction of autophagic cell death in human ovarian carcinoma cells by Antrodia salmonea through increased reactive oxygen species generation.
Ford MJ et al. Dev Cell 2018; 47 (4):509-523.e5 A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.
Tsai TL et al. J Virol 2018; (Epub) Interplay between the poly(A) tail, poly(A)-binding protein and coronavirus nucleocapsid protein regulates gene expression of the coronavirus and host cell.
Application : WB
Hu CM et al. FASEB J 2018;:fj201800856R Thymidylate kinase is critical for DNA repair via ATM-dependent Tip60 complex formation.
Application : ICC/IF
Cheng C et al. Hum Mol Genet 2018; (Epub) The long noncoding RNA NEAT1 is elevated in polyglutamine repeat expansion diseases and protects from disease-gene dependent toxicities.
Application : WB
Lai CY et al. Oncogene 2018; (Epub) Identification of UAP1L1 as a critical factor for protein O-GlcNAcylation and cell proliferation in human hepatoma cells.
Application : ICC/IF
Koba S et al. J Physiol 2018; (Epub) Sympathoexcitation by hypothalamic paraventricular nucleus neurons projecting to the rostral ventrolateral medulla.
Application : ICC/IF
Meriin AB et al. Proc Natl Acad Sci U S A 2018; (Epub) Hsp70-Bag3 complex is a hub for proteotoxicity-induced signaling that controls protein aggregation.
Schillemans M et al. Arterioscler Thromb Vasc Biol 2018; (Epub) The Weibel-Palade Body Localized SNARE (Soluble NSF Attachment Protein Receptor) Syntaxin-3 Modulates Von Willebrand Factor Secretion From Endothelial Cells.
Application : WB
Shukla S et al. Methods Mol Biol 2018; 1776:61-84 In Planta Production of Fluorescent Filamentous Plant Virus-Based Nanoparticles.
Bondar VV et al. Hum Mol Genet 2018; (Epub) PAK1 regulates ATXN1 levels providing an opportunity to modify its toxicity in Spinocerebellar ataxia type 1.
Application : WB
Xu Z et al. Cell Death Dis 2018; 9 (5):559 Checkpoint suppressor 1 suppresses transcriptional activity of ERα and breast cancer cell proliferation via deacetylase SIRT1.
Dodson M et al. Mol Cell Biol 2018; (Epub) Increased O-GlcNAcylation of SNAP29 drives arsenic-induced autophagic dysfunction.
Borga C et al. bioRxiv 2018; (Epub) Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis.
Application : IHC-P
Chou YT et al. J Biomed Sci 2018; 25 (1):6 Identification of as an isoform-specific inhibitor of HEME OXYGENASE-1/cytochrome P450 reductase interaction with anti-tumor activity.
Application : WB
Zhu Y et al. J Cell Biochem 2018; (Epub) Autophagy inhibition promotes phagocytosis of macrophage and protects mice from methicillin-resistant staphylococcus aureus pneumonia.
Application : ICC/IF
Lu JW et al. Blood Cancer J 2017; 7 (8):e593 Overexpression of SOX4 correlates with poor prognosis of acute myeloid leukemia and is leukemogenic in zebrafish.
Vega-Cabrera LA et al. PLoS One 2017; 12 (2):e0172737 Analysis of Spo0M function in Bacillus subtilis.
Gao Z et al. Development 2017; (Epub) PDGFRα / PDGFRβ signaling balance modulates progenitor cell differentiation into white and beige adipocytes.
Tzeng YW et al. Int J Biochem Cell Biol 2017; 94:22-30 LMO7 exerts an effect on mitosis progression and the spindle assembly checkpoint.
Application : WB, IP
van Ijzendoorn DGP et al. J Biol Chem 2017; (Epub) Functional Analyses of a Human Vascular Tumor FOS Variant Identify a Novel Degradation Mechanism and a link to Tumorigenesis.
Suboti? A et al. G3 (Bethesda) 2017; 7 (10):3509-3520 A Bimolecular Fluorescence Complementation Tool for Identification of Protein-Protein Interactions in Candida albicans.
Application : WB
Chang CT et al. Food Chem Toxicol 2017; 103:43117 Inhibition of ROS production, autophagy or apoptosis signaling reversed the anticancer properties of Antrodia salmonea in triple-negative breast cancer (MDA-MB-231) cells.
Application : ICC/IF
Solis GP et al. Cell 2017; (Epub) Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.
Application : WB
Abiria SA et al. Proc Natl Acad Sci U S A 2017; TRPM7 senses oxidative stress to release Zn2+ from unique intracellular vesicles.
Application : IP
Tillotson J et al. Chem Res Toxicol 2017; 30 (7):1508-1514 Arsenic Compromises Both p97 and Proteasome Functions.
Application : WB
Wu SC et al. Nat Commun 2017; 8 (1):24 Intestinal microbial dysbiosis aggravates the progression of Alzheimer's disease in Drosophila.
Chang CT et al. Arch Toxicol 2017; (Epub) Chalcone flavokawain B induces autophagic-cell death via reactive oxygen species-mediated signaling pathways in human gastric carcinoma and suppresses tumor growth in nude mice.
Application : WB
Harder B et al. Mol Carcinog 2016; (Epub) Brusatol overcomes chemoresistance through inhibition of protein translation.
Nyman JS et al. Bone 2016; 91:81-91 combined treatment with a transforming growth factor beta inhibitor (1d11) and * improves bone architecture in a mouse model of myeloma-induced bone disease.
Application : IHC-P
Koizumi H et al. Dev Neurobiol 2016; DCLK1 phosphorylates the microtubule-associated protein MAP7D1 to promote axon elongation in cortical neurons.
Wong MT et al. J Virol 2016; Human Choline Kinase-α Promotes Hepatitis C Virus RNA Replication through Modulating Membranous Viral Replication Complex Formation.
Application : WB
Lai CW et al. Mol Imaging Biol 2016; (Epub) Using Dual Fluorescence Reporting Genes to Establish an In Vivo Imaging Model of Orthotopic Lung Adenocarcinoma in Mice.
Application : WB, IHC-P
Lai CW et al. Sci Rep 2016; 6:24023 Sexually Dimorphic Expression of eGFP Transgene in the Akr1A1 Locus of Mouse Liver Regulated by Sex Hormone-Related Epigenetic Remodeling.
Application : WB, IHC-P
Xu Q et al. BMC Evol Biol 2016; 16 (1):11 Analysis of the erythropoietin of a Tibetan Plateau schizothoracine fish (Gymnocypris dobula) reveals enhanced cytoprotection function in hypoxic environments.
Yu H et al. Biosci Rep 2015; 35 (3) Autonomous and non-autonomous roles of DNase-II during cell death in C. elegans embryos.
Application : IHC-Wm
Steinke KV et al. J Biol Chem 2015; 290 (28):17390-400 Human CLC-K Channels Require Palmitoylation of Their Accessory Subunit Barttin to Be Functional.
Application : IP
Ross E et al. PLoS One 2015; 10 (5):e0128013 AMP-Activated Protein Kinase Regulates the Cell Surface Proteome and Integrin Membrane Traffic.
Application : WB
Yang FM et al. Biochem J 2015; 471 (2):199-209 A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding.
Application : IP
Dickmeis C et al. Front Bioeng Biotechnol 2015; 3:189 Production of Hybrid Chimeric PVX Particles Using a Combination of TMV and PVX-Based Expression Vectors.
Application : WB
Chen PJ et al. Nucleic Acids Res 2015; 43 (19):9393-404 NPGPx modulates CPEB2-controlled HIF-1α RNA translation in response to oxidative stress.
Li Q et al. J Biol Chem 2015; 290 (35):21553-67 Akt Phosphorylates Wnt Coactivator and Chromatin Effector Pygo2 at Serine 48 to Antagonize Its Ubiquitin/Proteasome-mediated Degradation.
Wu JM et al. Circ Res 2015; 116 (4):633-41 Circulating cells contribute to cardiomyocyte regeneration after injury.
Application : IHC-P
Lee WY et al. Oncotarget 2015; (Epub) repositioning antipsychotic * for treating colorectal cancer by inhibiting sirtuin 1.
Application : WB
Chen CJ et al. J Biol Chem 2015; 290 (10):6010-21 SH2B1 and IRSp53 Proteins Promote the Formation of Dendrites and Dendritic Branches.
Application : WB, ICC/IF
Wu HC et al. Biotechnol Prog 2015; Production and characterization of active recombinant interleukin-12/eGFP fusion protein in stably-transfected DF1 chicken cells.
Application : ELISA
Auer TO et al. Genome Res 2014; 24 (1):142-53 Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair.
Application : IHC-Wm
Hsueh YC et al. EMBO Mol Med 2014; 6 (4):496-503 Prostaglandin E- promotes post-infarction cardiomyocyte replenishment by endogenous stem cells.
Application : IHC-P
Willemen HL et al. J Pain 2014; 15 (5):496-506 Monocytes/Macrophages control resolution of transient inflammatory pain.
Application : IHC-Fr
Hong JB et al. PLoS One 2014; 9 (2):e89396 A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells.
Application : WB
Pope-Varsalona H et al. Nucleic Acids Res 2014; 42 (21):13096-109 Polymerase η suppresses telomere defects induced by DNA damaging agents.
Application : ICC/IF
Huang IH et al. J Cell Sci. 2014; 127 (Pt 19):4186-200 GEF-H1 controls focal adhesion signaling that regulates mesenchymal stem cell lineage commitment.
Application : WB
Yu Z et al. Cell Signal 2014; 26 (7):1560-1566 Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1α in HepG2 cells.
Application : WB
S Shukla et al. Biomater Sci 2014; Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models
Hsu CY et al. PLoS Pathog 2014; 10 (2):e1003974 The Epstein-Barr Virus-Encoded MicroRNA MiR-BART9 Promotes Tumor Metastasis by Targeting E-Cadherin in Nasopharyngeal Carcinoma.
Application : IHC
Chang YJ et al. Mol Cell Biol 2014; 34 (6):1003-19 SH2B1β Interacts with STAT3 and Enhances Fibroblast Growth Factor 1-Induced Gene Expression during Neuronal Differentiation.
Application : WB, IP
Mace PD et al. Nat Commun 2013; 4:1681 Structure of ERK2 bound to PEA-15 reveals a mechanism for * of activated MAPK.
Application : WB
Reetz J et al. PLoS One 2013; 8 (6):e67091 Development of Adenoviral Delivery Systems to Target Hepatic Stellate Cells In Vivo.
Application : IHC-P
Molleken K et al. PLoS Pathog 2013; 9 (4):e1003325 The Chlamydia pneumoniae invasin protein Pmp21 recruits the EGF receptor for host cell entry.
Wirth A et al. Biochem J 2013; 456 (3):311-22 Dual lipidation of the brain-specific Cdc42 isoform regulates its functional properties.
Barak LS et al. Biochemistry 2013; 52 (32):5403-14 Triphenylmethane dye activation of beta-arrestin.
Chang RY et al. Vet Microbiol 2013; 166 (43102):43425 japanese encephalitis virus non-coding rna inhibits activation of * by blocking nuclear translocation of * regulatory factor 3.
Application : IP
Park J et al. Nature 2013; 498 (7454):325-31 RAS-MAPK-MSK1 pathway modulates ataxin 1 protein levels and toxicity in SCA1.
Application : WB
Lin LT et al. J Biomed Opt 2013; 18 (12):126013 mPlum-IFP 1.4 fluorescent fusion protein may display F?rster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging.
Application : WB
Wang H et al. J Clin Invest 2013; 123 (12):5023-34 Balancing GRK2 and EPAC1 levels prevents and relieves chronic pain.
Application : IHC-Fr
He G et al. Cell 2013; 155 (2):384-96 Identification of liver cancer progenitors whose malignant progression depends on autocrine IL-6 signaling.
Application : IHC-P
Chiang YJ et al. PLoS One 2013; 8 (4):e61761 CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration.
Application : WB
Tsou TC et al. Toxicol Appl Pharmacol 2013; 273 (3):492-9 Crucial role of Toll-like receptors in the zinc/nickel-induced inflammatory response in vascular endothelial cells.
Application : WB
Hsu WB et al. J Virol 2013; 87 (1):110-23 Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation.
Application : WB
Wei PC et al. Mol Cell 2012; 48 (5):747-59 Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease.
Application : WB
Lai SL et al. Development 2012; 139 (23):4439-48 Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish.
Hsiao WC et al. Mol Pharm 2012; 9 (5):1396-408 Vitamin D3-inducible mesenchymal stem cell-based delivery of conditionally replicating adenoviruses effectively targets renal cell carcinoma and inhibits tumor growth.
Application : IHC
Kwanchanok S et al. Biosci Biotechnol Biochem 2011; 75 (10):37073 In vitro fluorescent analysis of preprotein import into chloroplasts.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169