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GFP antibody

Overexpression
Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617

Cat No. GTX113617

Reference 120
20

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, Dot, ELISA, IHC
Package
100 μl ($329),
25 μl ($169)
See all GFP products
PRODUCT

Summary

GFP antibody recognizes the green fluorescent protein (GFP), a fluorophore in widespread use in biomedical research. GFP fluorescence allows real-time study of physiological processes involving fusion proteins or GFP-expressing cells. This antibody also detects other GFP variants, including EGFP and YFP, but not red fluorescent protein (RFP).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:1000-1:5000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IP 1:100-1:500
Dot Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

human GFP-transfected 293T , 293T whole cell extract expressing GFP-tagged protein , *GFP-p53 transfected U2OS

Product Note

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950371

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Green Fluorescent Protein , GFP , eGFP , enhanced green fluorescent protein

Research Area

DATA IMAGES

Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617

GTX113617 IHC-Wm Image

Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo (fixed with ice-cold acetone), using GFP(GTX113617) antibody at 1:200 dilution.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617

GTX113617 IP Image

GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
The precipitated GFP was detected by GTX113617 diluted at 1:15000.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis.
A. 1 μg 293T whole cell extract
B. 1 μg 293T whole cell extract expressing GFP-tagged protein
C. 1 μg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX113617) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617

GTX113617 ICC/IF Image

Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

GFP antibody detects GFP protein by Western blot analysis. Non-transfected (-) and GFP-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) diluted at 1:10000.

REFERENCE

Application Reference

Chang YC et al. Nat Commun 2021; 12 (1) : 4258 VCP maintains nuclear size by regulating the DNA damage-associated MDC1-p53-autophagy axis in Drosophila.
Application : WB
Hsieh YC et al. Cell Mol Gastroenterol Hepatol 2021; 12 (3) : 813-838 (Pro)renin Receptor Knockdown Attenuates Liver Fibrosis Through Inactivation of ERK/TGF-β1/SMAD3 Pathway.
Application : IHC
Miki T et al. Nat Commun 2021; 12 (1) : 3412 Intracellular artificial supramolecules based on de novo designed Y15 peptides.
Application : WB
Ou M et al. Oncol Rep 2021; 46 (1) MDM2 induces EMT via the B?Raf signaling pathway through 14?3?3.
Application : WB
Li X et al. NPJ Precis Oncol 2021; 5 (1) : 20 Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1.
Application : WB, IP
Show More +
Submit a Reference

REVIEW

GFP antibody Cat No. GTX113617

Rating
( Average 5 based on 20 users reviews)
Application
Western Blot(WB)
( Average 4.9 based on 15 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 2 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-GFP antibody used in IHC (Paraffin sections) (IHC-P). GTX113617
Date : Anonymous submitted on 31-Aug-2021
Score :
Application Tested : IHC-P
Sample : mFLE-EGFP mouse testis, 2-dpp, paraffin-section 5μm
Blocking : 5% donkey-serum in TNB, 0.3% Triton X-100, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : The 2-dpp testis tissue section of mFLE-EGFP transgenic mouse which present fetal Leydig cell (fLC) was stained by GFP antibody and HSD-3b1 antibody. GFP only expressed in fLC.
Anti-GFP antibody used in IHC (Frozen sections) (IHC-Fr). GTX113617
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018.PMID: 30310071
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2018.PMID: 29361943
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IP,WB
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018.PMID: 29752474
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018.PMID: 29752474
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018.PMID: 29752474
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Figure Note : The data was peer reviewed and published in the journal J Virol in 2016.PMID: 27489281
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Adv Sci (Weinh) in 2020.PMID: 32195080
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Commun Biol in 2019.PMID: 31754649
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Commun Biol in 2019.PMID: 31754649
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Commun Biol in 2019.PMID: 31754649
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019.PMID: 31311575
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019.PMID: 31311575
Anti-GFP antibody used in IHC (Paraffin sections) (IHC-P). GTX113617
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2014.PMID: 24448489
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 18-Oct-2018
Score :
Application Tested : WB
Sample : BY4741 (budding yeast) expressing GFP(S65T)-tagged Vps21p (small GTPase Rab5 homologue)
Amount used : 5 μg
Blocking : 1% nonfat milk in PBS-0.05% Tween-20, 25°C, 0.5Hr
Primary Antibody : 1:10000, 4°C, 5Hr
Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617
Date : Anonymous submitted on 01-Jun-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila eye
Fixation : 4% PFA, 4°C, 0.17Hr
Permeabilization /Antigen retrieval : 0.3% PBST, 10 minHr
Primary Antibody : 1:500, 4°C, 16Hr
Anti-GFP antibody used in Immunohistochemistry (IHC). GTX113617
Date : Anonymous submitted on 22-Feb-2018
Score :
Application Tested : IHC
Sample : Drosophila Retinal tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Green: GTX113617 GFP antibody: photoreceptor cell R8, Red: GTX630189 mcherry antibody: medulla
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP recombinant protein transfected 293T cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP-p53 transfected U2OS cells
Amount used : 15μg
Blocking : 5% milk in 0.1%TBST, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : A: U2OS cells transfected with GFP-p53, B: U2OS cells transfected with control plasmid (no GFP-tag)
Anti-GFP antibody used in Western Blot (WB). GTX113617
Date : Anonymous submitted on 12-Apr-2012
Score :
Application Tested : WB
Sample : Protoplast from Rosette leaves
Amount used : 30μg
Blocking : 5% milk in PBST, 4°C, 16Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Show More +
Submit a Review
Application Reference
Chang YC et al. Nat Commun 2021; 12 (1):4258 VCP maintains nuclear size by regulating the DNA damage-associated MDC1-p53-autophagy axis in Drosophila.
Application : WB
Hsieh YC et al. Cell Mol Gastroenterol Hepatol 2021; 12 (3):813-838 (Pro)renin Receptor Knockdown Attenuates Liver Fibrosis Through Inactivation of ERK/TGF-β1/SMAD3 Pathway.
Application : IHC
Miki T et al. Nat Commun 2021; 12 (1):3412 Intracellular artificial supramolecules based on de novo designed Y15 peptides.
Application : WB
Ou M et al. Oncol Rep 2021; 46 (1) MDM2 induces EMT via the B?Raf signaling pathway through 14?3?3.
Application : WB
Li X et al. NPJ Precis Oncol 2021; 5 (1):20 Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1.
Application : WB, IP
Li B et al. Cancer Cell Int 2021; 21 (1):38 TIMELESS inhibits breast cancer cell invasion and metastasis by down-regulating the expression of MMP9.
Application : WB
Fan Z et al. Antimicrob Agents Chemother 2021; 65 (2) Pseudomonas aeruginosa Polynucleotide Phosphorylase Controls Tolerance to Aminoglycoside Antibiotics by Regulating the MexXY Multidrug Efflux Pump.
Application : WB
Yin D et al. Nat Biotechnol 2021; Targeting herpes simplex virus with CRISPR-Cas9 cures herpetic stromal keratitis in mice.
Application : IHC-Fr
Huang YL et al. Elife 2020; 9 Collagen-rich omentum is a premetastatic niche for integrin α2-mediated peritoneal metastasis.
Chang YJ et al. Cancers (Basel) 2020; 12 (10) Mitochondrial ROS1 Increases Mitochondrial Fission and Respiration in Oral Squamous Cancer Carcinoma.
Fuchigami T et al. J Appl Oral Sci 2020; 28:e20190558 Ameloblastoma cell lines derived from different subtypes demonstrate distinct developmental patterns in a novel animal experimental model.
Young TJ et al. PLoS Genet 2020; 16 (12):e1009226 CAF-1 and Rtt101p function within the replication-coupled chromatin assembly network to promote H4 K16ac, preventing ectopic silencing.
Application : WB
Kumar R et al. Adv Sci (Weinh) 2020; 7 (6):1902372 Fibroblast Growth Factor 2-Mediated Regulation of Neuronal Exosome Release Depends on VAMP3/Cellubrevin in Hippocampal Neurons.
Hao P et al. Life Sci 2020; 262:118492 Lipocalin2 promotes cell proliferation and migration in ovarian cancer through activation of the ERK/GSK3β/β-catenin signaling pathway.
Application : WB
Dai YC et al. J Virol 2020; 94 (3) The Novel Nuclear Targeting and BFRF1-Interacting Domains of BFLF2 Are Essential for Efficient Epstein-Barr Virus Virion Release.
Meng J et al. Small 2020; 16 (31):e2001890 Synthetic CXCR4 Antagonistic Peptide Assembling with Nanoscaled Micelles Combat Acute Myeloid Leukemia.
Wu AY et al. Adv Sci (Weinh) 2020; 7 (19):2001467 Multiresolution Imaging Using Bioluminescence Resonance Energy Transfer Identifies Distinct Biodistribution Profiles of Extracellular Vesicles and Exomeres with Redirected Tropism.
Application : Dot
Seguin A et al. J Biol Chem 2020; 295 (32):11002-11020 The mitochondrial metal transporters mitoferrin1 and mitoferrin2 are required for liver regeneration and cell proliferation in mice.
Tsai HY et al. Dis Model Mech 2020; 13 (8) Loss of the Drosophila branched-chain α-ketoacid dehydrogenase complex results in neuronal dysfunction.
Application : WB
Bernadzki KM et al. Front Mol Neurosci 2020; 13:104 Arhgef5 Binds α-Dystrobrevin 1 and Regulates Neuromuscular Junction Integrity.
Application : IP
Fukushima Y et al. EMBO J 2020; 39 (12):e102930 RhoJ integrates attractive and repulsive cues in directional migration of endothelial cells.
Application : WB, IHC-Wm
Wang IH et al. Cancers (Basel) 2020; 12 (5) Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression.
Application : WB
Seiya Yamada et al. iScience 2020; 23 (5):101058 Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.
Application : IHC-Fr
Anthony Yan-Tang Wu et al. bioRxiv 2020; (Epub) Redirected tropisms of extracellular vesicles and exomeres yield distinct biodistribution profiles.
Pei YF et al. Am J Pathol 2020; 190 (6):1343-1354 The Crosstalk between lncRNA-SNHG7/miRNA-181/cbx7 Modulates Malignant Character in Lung Adenocarcinoma.
Application : WB
Chen X et al. Hum Gene Ther 2020; 31 (7-8):440-447 Different Serotypes of Adeno-Associated Virus Vector- and Lentivirus-Mediated Tropism in Choroid Plexus by Intracerebroventricular Delivery.
Application : ICC/IF
Chen CH et al. Biochem Biophys Res Commun 2020; 524 (4):1003-1009 Dicoumarol suppresses HMGA2-mediated oncogenic capacities and inhibits cell proliferation by inducing apoptosis in colon cancer.
Application : WB
Iwaizumi M et al. Fish Physiol Biochem 2020; (Epub) Plasmid delivery by electroporation into fish skeletal muscle for recombinant protein secretion and uptake by oocytes.
Application : IHC-P
Rohit Kumar et al. Advanced Science 2020; (Epub) Fibroblast Growth Factor 2?Mediated Regulation of Neuronal Exosome Release Depends on VAMP3/Cellubrevin in Hippocampal Neurons.
Akiyama H et al. Cell Tissue Res 2020; (Epub) Control of cell migration by the novel protein phosphatase-2A interacting protein inka2.
Application : WB
Sarkar A et al. Haematologica 2020; (Epub) Ataxia telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma.
Application : WB
Chen PH et al. FASEB J 2020; 34 (1):1107-1121 Nucleolar control by a non-apoptotic p53-caspases-deubiquitinylase axis promotes resistance to bacterial infection.
Application : WB
Nagano M et al. Commun Biol 2019; 2 (1):419 Rab5-mediated endosome formation is regulated at the trans-Golgi network.
Application : WB
Cassidy KB et al. FEBS J 2019; (Epub) Direct regulation of Chk1 protein stability by E3 ubiquitin ligase HUWE1.
Application : WB
Ke YT et al. G3 (Bethesda) 2019; 9 (6):2007-2016 Generation of Inducible Gene-Switched GAL4 Expressed in the Drosophila Female Germline Stem Cell Niche.
Nagano M et al. Commun Biol 2019; 2:419 Rab5-mediated endosome formation is regulated at the trans-Golgi network.
Application : WB
Ngo AM et al. Elife 2019; 8 The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
Application : WB
Zanin R et al. J Exp Clin Cancer Res 2019; 38 (1):313 HMGA1 promotes breast cancer angiogenesis supporting the stability, nuclear localization and transcriptional activity of FOXM1.
Application : IP
Shull TE et al. Environ Sci Technol 2019; 53 (16):9522-9532 Anatase TiO2 Nanoparticles Induce Autophagy and Chloroplast Degradation in Thale Cress (Arabidopsis thaliana).
Application : WB
Chang CH et al. Cell Rep 2019; 28 (5):1195-1205.e3 The Ventral Hippocampus Controls Stress-Provoked Impulsive Aggression through the Ventromedial Hypothalamus in Post-Weaning Social Isolation Mice.
Application : IHC-Fr
Lai TC et al. Sci Rep 2019; 9 (1):5304 Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism.
Application : WB
Fern?ndez MR et al. Methods Mol Biol 2019; 1958:237-261 Formation of Cross-Beta Supersecondary Structure by Soft-Amyloid Cores: Strategies for Their Prediction and Characterization.
Li H et al. Int J Med Sci 2019; 16 (1):106-114 MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation.
Kozareva DA et al. Behav Brain Res 2019; 360:36-50 TLX knockdown in the dorsal dentate gyrus of juvenile rats differentially affects adolescent and adult behaviour.
Application : IHC-Fr
Tanaka J et al. Nat Commun 2018; 9 (1):4216 Generation of orthotopically functional salivary gland from embryonic stem cells.
Yang HL et al. J Cell Physiol 2018; (Epub) Induction of autophagic cell death in human ovarian carcinoma cells by Antrodia salmonea through increased reactive oxygen species generation.
Ford MJ et al. Dev Cell 2018; 47 (4):509-523.e5 A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.
Tsai TL et al. J Virol 2018; (Epub) Interplay between the poly(A) tail, poly(A)-binding protein and coronavirus nucleocapsid protein regulates gene expression of the coronavirus and host cell.
Application : WB
Hu CM et al. FASEB J 2018;:fj201800856R Thymidylate kinase is critical for DNA repair via ATM-dependent Tip60 complex formation.
Application : ICC/IF
Cheng C et al. Hum Mol Genet 2018; (Epub) The long noncoding RNA NEAT1 is elevated in polyglutamine repeat expansion diseases and protects from disease-gene dependent toxicities.
Application : WB
Lai CY et al. Oncogene 2018; (Epub) Identification of UAP1L1 as a critical factor for protein O-GlcNAcylation and cell proliferation in human hepatoma cells.
Application : ICC/IF
Koba S et al. J Physiol 2018; (Epub) Sympathoexcitation by hypothalamic paraventricular nucleus neurons projecting to the rostral ventrolateral medulla.
Application : ICC/IF
Meriin AB et al. Proc Natl Acad Sci U S A 2018; (Epub) Hsp70-Bag3 complex is a hub for proteotoxicity-induced signaling that controls protein aggregation.
Schillemans M et al. Arterioscler Thromb Vasc Biol 2018; (Epub) The Weibel-Palade Body Localized SNARE (Soluble NSF Attachment Protein Receptor) Syntaxin-3 Modulates Von Willebrand Factor Secretion From Endothelial Cells.
Application : WB
Shukla S et al. Methods Mol Biol 2018; 1776:61-84 In Planta Production of Fluorescent Filamentous Plant Virus-Based Nanoparticles.
Bondar VV et al. Hum Mol Genet 2018; (Epub) PAK1 regulates ATXN1 levels providing an opportunity to modify its toxicity in Spinocerebellar ataxia type 1.
Application : WB
Xu Z et al. Cell Death Dis 2018; 9 (5):559 Checkpoint suppressor 1 suppresses transcriptional activity of ERα and breast cancer cell proliferation via deacetylase SIRT1.
Dodson M et al. Mol Cell Biol 2018; (Epub) Increased O-GlcNAcylation of SNAP29 drives arsenic-induced autophagic dysfunction.
Borga C et al. bioRxiv 2018; (Epub) Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis.
Application : IHC-P
Chou YT et al. J Biomed Sci 2018; 25 (1):6 Identification of as an isoform-specific inhibitor of HEME OXYGENASE-1/cytochrome P450 reductase interaction with anti-tumor activity.
Application : WB
Zhu Y et al. J Cell Biochem 2018; (Epub) Autophagy inhibition promotes phagocytosis of macrophage and protects mice from methicillin-resistant staphylococcus aureus pneumonia.
Application : ICC/IF
Xie WR et al. J Neurosci 2017; 37 (36):8583-8594 An Atoh1-S193A Phospho-Mutant Allele Causes Hearing Deficits and Motor Impairment.
Lu JW et al. Blood Cancer J 2017; 7 (8):e593 Overexpression of SOX4 correlates with poor prognosis of acute myeloid leukemia and is leukemogenic in zebrafish.
Vega-Cabrera LA et al. PLoS One 2017; 12 (2):e0172737 Analysis of Spo0M function in Bacillus subtilis.
Gao Z et al. Development 2017; (Epub) PDGFRα / PDGFRβ signaling balance modulates progenitor cell differentiation into white and beige adipocytes.
Tzeng YW et al. Int J Biochem Cell Biol 2017; 94:22-30 LMO7 exerts an effect on mitosis progression and the spindle assembly checkpoint.
Application : WB, IP
van Ijzendoorn DGP et al. J Biol Chem 2017; (Epub) Functional Analyses of a Human Vascular Tumor FOS Variant Identify a Novel Degradation Mechanism and a link to Tumorigenesis.
Suboti? A et al. G3 (Bethesda) 2017; 7 (10):3509-3520 A Bimolecular Fluorescence Complementation Tool for Identification of Protein-Protein Interactions in Candida albicans.
Application : WB
Chang CT et al. Food Chem Toxicol 2017; 103:43117 Inhibition of ROS production, autophagy or apoptosis signaling reversed the anticancer properties of Antrodia salmonea in triple-negative breast cancer (MDA-MB-231) cells.
Application : ICC/IF
Solis GP et al. Cell 2017; (Epub) Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.
Application : WB
Abiria SA et al. Proc Natl Acad Sci U S A 2017; TRPM7 senses oxidative stress to release Zn2+ from unique intracellular vesicles.
Application : IP
Tillotson J et al. Chem Res Toxicol 2017; 30 (7):1508-1514 Arsenic Compromises Both p97 and Proteasome Functions.
Application : WB
Wu SC et al. Nat Commun 2017; 8 (1):24 Intestinal microbial dysbiosis aggravates the progression of Alzheimer's disease in Drosophila.
Chang CT et al. Arch Toxicol 2017; (Epub) Chalcone flavokawain B induces autophagic-cell death via reactive oxygen species-mediated signaling pathways in human gastric carcinoma and suppresses tumor growth in nude mice.
Application : WB
Harder B et al. Mol Carcinog 2016; (Epub) Brusatol overcomes chemoresistance through inhibition of protein translation.
Nyman JS et al. Bone 2016; 91:81-91 combined treatment with a transforming growth factor beta inhibitor (1d11) and * improves bone architecture in a mouse model of myeloma-induced bone disease.
Application : IHC-P
Koizumi H et al. Dev Neurobiol 2016; DCLK1 phosphorylates the microtubule-associated protein MAP7D1 to promote axon elongation in cortical neurons.
Wong MT et al. J Virol 2016; Human Choline Kinase-α Promotes Hepatitis C Virus RNA Replication through Modulating Membranous Viral Replication Complex Formation.
Application : WB
Lai CW et al. Mol Imaging Biol 2016; (Epub) Using Dual Fluorescence Reporting Genes to Establish an In Vivo Imaging Model of Orthotopic Lung Adenocarcinoma in Mice.
Application : WB, IHC-P
Lai CW et al. Sci Rep 2016; 6:24023 Sexually Dimorphic Expression of eGFP Transgene in the Akr1A1 Locus of Mouse Liver Regulated by Sex Hormone-Related Epigenetic Remodeling.
Application : WB, IHC-P
Xu Q et al. BMC Evol Biol 2016; 16 (1):11 Analysis of the erythropoietin of a Tibetan Plateau schizothoracine fish (Gymnocypris dobula) reveals enhanced cytoprotection function in hypoxic environments.
Wu HH et al. J Exp Med 2015; 212 (3):333-49 Targeting IL-17B-IL-17RB signaling with an anti-IL-17RB antibody blocks pancreatic cancer metastasis by silencing multiple chemokines.
Yu H et al. Biosci Rep 2015; 35 (3) Autonomous and non-autonomous roles of DNase-II during cell death in C. elegans embryos.
Application : IHC-Wm
Steinke KV et al. J Biol Chem 2015; 290 (28):17390-400 Human CLC-K Channels Require Palmitoylation of Their Accessory Subunit Barttin to Be Functional.
Application : IP
Ross E et al. PLoS One 2015; 10 (5):e0128013 AMP-Activated Protein Kinase Regulates the Cell Surface Proteome and Integrin Membrane Traffic.
Application : WB
Yang FM et al. Biochem J 2015; 471 (2):199-209 A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding.
Application : IP
Dickmeis C et al. Front Bioeng Biotechnol 2015; 3:189 Production of Hybrid Chimeric PVX Particles Using a Combination of TMV and PVX-Based Expression Vectors.
Application : WB
Chen PJ et al. Nucleic Acids Res 2015; 43 (19):9393-404 NPGPx modulates CPEB2-controlled HIF-1α RNA translation in response to oxidative stress.
Li Q et al. J Biol Chem 2015; 290 (35):21553-67 Akt Phosphorylates Wnt Coactivator and Chromatin Effector Pygo2 at Serine 48 to Antagonize Its Ubiquitin/Proteasome-mediated Degradation.
Wu JM et al. Circ Res 2015; 116 (4):633-41 Circulating cells contribute to cardiomyocyte regeneration after injury.
Application : IHC-P
Lee WY et al. Oncotarget 2015; (Epub) repositioning antipsychotic * for treating colorectal cancer by inhibiting sirtuin 1.
Application : WB
Chen CJ et al. J Biol Chem 2015; 290 (10):6010-21 SH2B1 and IRSp53 Proteins Promote the Formation of Dendrites and Dendritic Branches.
Application : WB, ICC/IF
Wu HC et al. Biotechnol Prog 2015; Production and characterization of active recombinant interleukin-12/eGFP fusion protein in stably-transfected DF1 chicken cells.
Application : ELISA
Auer TO et al. Genome Res 2014; 24 (1):142-53 Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair.
Application : IHC-Wm
Hsueh YC et al. EMBO Mol Med 2014; 6 (4):496-503 Prostaglandin E- promotes post-infarction cardiomyocyte replenishment by endogenous stem cells.
Application : IHC-P
Willemen HL et al. J Pain 2014; 15 (5):496-506 Monocytes/Macrophages control resolution of transient inflammatory pain.
Application : IHC-Fr
Hong JB et al. PLoS One 2014; 9 (2):e89396 A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells.
Application : WB
Pope-Varsalona H et al. Nucleic Acids Res 2014; 42 (21):13096-109 Polymerase η suppresses telomere defects induced by DNA damaging agents.
Application : ICC/IF
Huang IH et al. J Cell Sci. 2014; 127 (Pt 19):4186-200 GEF-H1 controls focal adhesion signaling that regulates mesenchymal stem cell lineage commitment.
Application : WB
Yu Z et al. Cell Signal 2014; 26 (7):1560-1566 Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1α in HepG2 cells.
Application : WB
S Shukla et al. Biomater Sci 2014; Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models
Hsu CY et al. PLoS Pathog 2014; 10 (2):e1003974 The Epstein-Barr Virus-Encoded MicroRNA MiR-BART9 Promotes Tumor Metastasis by Targeting E-Cadherin in Nasopharyngeal Carcinoma.
Application : IHC
Chang YJ et al. Mol Cell Biol 2014; 34 (6):1003-19 SH2B1β Interacts with STAT3 and Enhances Fibroblast Growth Factor 1-Induced Gene Expression during Neuronal Differentiation.
Application : WB, IP
Mace PD et al. Nat Commun 2013; 4:1681 Structure of ERK2 bound to PEA-15 reveals a mechanism for * of activated MAPK.
Application : WB
Reetz J et al. PLoS One 2013; 8 (6):e67091 Development of Adenoviral Delivery Systems to Target Hepatic Stellate Cells In Vivo.
Application : IHC-P
Molleken K et al. PLoS Pathog 2013; 9 (4):e1003325 The Chlamydia pneumoniae invasin protein Pmp21 recruits the EGF receptor for host cell entry.
Wirth A et al. Biochem J 2013; 456 (3):311-22 Dual lipidation of the brain-specific Cdc42 isoform regulates its functional properties.
Barak LS et al. Biochemistry 2013; 52 (32):5403-14 Triphenylmethane dye activation of beta-arrestin.
Chang RY et al. Vet Microbiol 2013; 166 (43102):43425 japanese encephalitis virus non-coding rna inhibits activation of * by blocking nuclear translocation of * regulatory factor 3.
Application : IP
Park J et al. Nature 2013; 498 (7454):325-31 RAS-MAPK-MSK1 pathway modulates ataxin 1 protein levels and toxicity in SCA1.
Application : WB
Lin LT et al. J Biomed Opt 2013; 18 (12):126013 mPlum-IFP 1.4 fluorescent fusion protein may display F?rster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging.
Application : WB
Wang H et al. J Clin Invest 2013; 123 (12):5023-34 Balancing GRK2 and EPAC1 levels prevents and relieves chronic pain.
Application : IHC-Fr
He G et al. Cell 2013; 155 (2):384-96 Identification of liver cancer progenitors whose malignant progression depends on autocrine IL-6 signaling.
Application : IHC-P
Chiang YJ et al. PLoS One 2013; 8 (4):e61761 CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration.
Application : WB
Tsou TC et al. Toxicol Appl Pharmacol 2013; 273 (3):492-9 Crucial role of Toll-like receptors in the zinc/nickel-induced inflammatory response in vascular endothelial cells.
Application : WB
Hsu WB et al. J Virol 2013; 87 (1):110-23 Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation.
Application : WB
Wei PC et al. Mol Cell 2012; 48 (5):747-59 Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease.
Application : WB
Lai SL et al. Development 2012; 139 (23):4439-48 Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish.
Hsiao WC et al. Mol Pharm 2012; 9 (5):1396-408 Vitamin D3-inducible mesenchymal stem cell-based delivery of conditionally replicating adenoviruses effectively targets renal cell carcinoma and inhibits tumor growth.
Application : IHC
Kwanchanok S et al. Biosci Biotechnol Biochem 2011; 75 (10):37073 In vitro fluorescent analysis of preprotein import into chloroplasts.
Application : WB
SDS
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