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GFP antibody

Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617

Cat No. GTX113617

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, Dot, ELISA, IHC

Reactivity

Drosophila
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

GFP antibody recognizes the green fluorescent protein (GFP), a fluorophore in widespread use in biomedical research. GFP fluorescence allows real-time study of physiological processes involving fusion proteins or GFP-expressing cells. This antibody also detects other GFP variants, including EGFP and YFP, but not red fluorescent protein (RFP).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:1000-1:5000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IP 1:100-1:500
Dot Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

human GFP-transfected 293T , 293T whole cell extract expressing GFP-tagged protein , *GFP-p53 transfected U2OS

Product Note

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950371

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Green Fluorescent Protein , GFP , eGFP , enhanced green fluorescent protein

Research Area

DATA IMAGES

Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617

GTX113617 IHC-Wm Image

Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo (fixed with ice-cold acetone), using GFP(GTX113617) antibody at 1:200 dilution.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617

GTX113617 IP Image

GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
The precipitated GFP was detected by GTX113617 diluted at 1:15000.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis.
A. 1 μg 293T whole cell extract
B. 1 μg 293T whole cell extract expressing GFP-tagged protein
C. 1 μg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX113617) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617

GTX113617 ICC/IF Image

Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

GFP antibody detects GFP protein by Western blot analysis. Non-transfected (-) and GFP-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) diluted at 1:10000.

REFERENCE

REVIEW

GFP antibody Cat No. GTX113617

Rating ( Average 5 based on 20 users reviews)
Application
Western Blot(WB) ( Average 4.9 based on 15 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 2 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 31-Aug-2021
Score :
Application Tested : IHC-P
Sample : mFLE-EGFP mouse testis, 2-dpp, paraffin-section 5μm
Blocking : 5% donkey-serum in TNB, 0.3% Triton X-100, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : The 2-dpp testis tissue section of mFLE-EGFP transgenic mouse which present fetal Leydig cell (fLC) was stained by GFP antibody and HSD-3b1 antibody. GFP only expressed in fLC.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018.PMID: 30310071
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2018.PMID: 29361943
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IP,WB
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018.PMID: 29752474
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018.PMID: 29752474
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018.PMID: 29752474
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Figure Note : The data was peer reviewed and published in the journal J Virol in 2016.PMID: 27489281
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Adv Sci (Weinh) in 2020.PMID: 32195080
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Commun Biol in 2019.PMID: 31754649
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Commun Biol in 2019.PMID: 31754649
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Commun Biol in 2019.PMID: 31754649
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019.PMID: 31311575
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019.PMID: 31311575
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2014.PMID: 24448489
Date : Anonymous submitted on 18-Oct-2018
Score :
Application Tested : WB
Sample : BY4741 (budding yeast) expressing GFP(S65T)-tagged Vps21p (small GTPase Rab5 homologue)
Amount used : 5 μg
Blocking : 1% nonfat milk in PBS-0.05% Tween-20, 25°C, 0.5Hr
Primary Antibody : 1:10000, 4°C, 5Hr
Date : Anonymous submitted on 01-Jun-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila eye
Fixation : 4% PFA, 4°C, 0.17Hr
Permeabilization /Antigen retrieval : 0.3% PBST, 10 minHr
Primary Antibody : 1:500, 4°C, 16Hr
Date : Anonymous submitted on 22-Feb-2018
Score :
Application Tested : IHC
Sample : Drosophila Retinal tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Green: GTX113617 GFP antibody: photoreceptor cell R8, Red: GTX630189 mcherry antibody: medulla
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP recombinant protein transfected 293T cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP-p53 transfected U2OS cells
Amount used : 15μg
Blocking : 5% milk in 0.1%TBST, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : A: U2OS cells transfected with GFP-p53, B: U2OS cells transfected with control plasmid (no GFP-tag)
Date : Anonymous submitted on 12-Apr-2012
Score :
Application Tested : WB
Sample : Protoplast from Rosette leaves
Amount used : 30μg
Blocking : 5% milk in PBST, 4°C, 16Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169