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GJB1 antibody [GJB1/1753]

Anti-GJB1 antibody [GJB1/1753] used in IHC (Paraffin sections) (IHC-P). GTX34760
Anti-GJB1 antibody [GJB1/1753] used in IHC (Paraffin sections) (IHC-P). GTX34760

Cat No. GTX34760

Host Mouse
Clonality Monoclonal
Clone Name GJB1/1753
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Specificity/Sensitivity

This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane α-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human GJB1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gap Junction Protein Beta 1,Cmtx,Cmtx1,Cx32,Gjb1

Background

This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2008]

Database

DATA IMAGES
Anti-GJB1 antibody [GJB1/1753] used in IHC (Paraffin sections) (IHC-P). GTX34760

GTX34760 IHC-P Image

Formalin-fixed, paraffin-embedded human Liver stained with Connexin 32 Monoclonal Antibody (GJB1/1753)

Anti-GJB1 antibody [GJB1/1753] used in IHC (Paraffin sections) (IHC-P). GTX34760

GTX34760 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with Connexin 32 Monoclonal Antibody (GJB1/1753)

Package List Price ($)
$ 329