GeneTex
United States (US)

GLE1 antibody

GTX31366_958_IHC-P_20180221_18121410_437.jpg
GTX31366_959_IHC-P_20180221_18121410_167.jpg
GTX31366_2566_WB_20180221_18121410_850.jpg

Cat No. GTX31366

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Mouse Brain Tissue Lysate(GTX27934)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Gle1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Gle1.The immunogen is located within amino acids 540 - 590 of Gle1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gle1, Rna Export Mediator,Gle1L,Lccs,Lccs1,Hgle1,Gle1

Background

This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX31366_958_IHC-P_20180221_18121410_437.jpg

GTX31366 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31366 GLE1 antibody.
Working concentration : 5 μg/ml

GTX31366_959_IHC-P_20180221_18121410_167.jpg

GTX31366 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31366 GLE1 antibody.
Working concentration : 2.5 μg/ml

GTX31366_2566_WB_20180221_18121410_850.jpg

GTX31366 WB Image

WB analysis of mouse brain tissue lysate using GTX31366 GLE1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Package List Price ($)
$ 329