This uses GTX60951 as capture antibody in combination with biotinylated detection antibody GTX60952 -02 exclusively to measure GLP-1(7-36)amide in biological samples. GLP-1(7-36)amide is the principal active form of the peptide, and has been estimated to make up about 70% of the physiological response. Relative to measuring both active forms of GLP-1 together, the responses are reduced by not measuring the approximately 30% contribution of GLP-1(7-37) to the overall response. There is no cross-reactivity with any extended or truncated physiological form of GLP-1(7-36)amide. Specimens must be collected into tubes containing an adequate amount of DPP-4 inhibitor (in addition to routine peptidase inhibitors) to prevent the rapid in vitro degradation of the active peptide.

The calibration curve of a sandwich assay for Non-amidated GLP-1 using GTX60954 as the capture antibody and GTX60952 -02 as the biotinylated detection antibody.