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GM130 antibody

Anti-GM130 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130351
Anti-GM130 antibody used in Immunoprecipitation (IP). GTX130351
Anti-GM130 antibody used in Western Blot (WB). GTX130351

Cat. No. GTX130351

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GM130 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GM130. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886243

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgin A2 , GM130

Cellular Localization

Golgi apparatus , Golgi stack membrane; Peripheral membrane protein

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GM130 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130351

GTX130351 ICC/IF Image

GM130 antibody detects GM130 protein at Golgi apparatus and endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GM130 protein stained by GM130 antibody (GTX130351) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-GM130 antibody used in Immunoprecipitation (IP). GTX130351

GTX130351 IP Image

Immunoprecipitation of GM130 protein from HeLa whole cell extracts using 5 μg of GM130 antibody (GTX130351).
Western blot analysis was performed using GM130 antibody (GTX130351).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GM130 antibody used in Western Blot (WB). GTX130351

GTX130351 WB Image

GM130 antibody detects GM130 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GM130 antibody (GTX130351) diluted by 1:1000.

REFERENCE

Application Reference

Viettri M et al. Sci Rep 2021; 11 (1) : 23489 Flavivirus infections induce a Golgi stress response in vertebrate and mosquito cells.
Application : ICC/IF
Lee HY et al. Oncotarget 2018; 9 (17) : 13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Application : WB
Reactivity : Human
Han H et al. Hepatology Communications 2017; (Epub) Disrupted ER-to-Golgi trafficking underlies anti-HIV drugs and alcohol-induced cellular stress and hepatic injury.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169