APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
H1299 , Raji , 293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GPI. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037119
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
glucose-6-phosphate isomerase , AMF , GNPI , NLK , PGI , PHI , SA-36 , SA36
Cellular Localization
Cytoplasm , Secreted
Background
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113203 WB Image
Wild-type (WT) and GPI knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPI antibody (GTX113203) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX113203 ICC/IF Image
GPI antibody detects GPI protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: GPI protein stained by GPI antibody (GTX113203) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX113203 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GPI(GTX113203) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113203 WB Image
Sample (30 ug of whole cell lysate)
A: H1299
B: Raji
7.5% SDS PAGE
GTX113203 diluted at 1:10000
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GTX113203 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPI antibody (GTX113203) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX113203 WB Image
Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX113203 diluted at 1:1000
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GTX113203 IHC-Fr Image
Immunohistochemical analysis of frozen sections of zebrafish retina, using GPI antibody (GTX113203) at 1:2000 dilution.
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GTX113203 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using GPI antibody (GTX113203) at 1:300 dilution.
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REFERENCE
REVIEW
GPI antibody
Cat. No. GTX113203
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Viruses in 2018.
PMID: 29518929
