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GPI antibody

KO/KD
Anti-GPI antibody used in Western Blot (WB). GTX113203
Anti-GPI antibody used in Western Blot (WB). GTX113203
Anti-GPI antibody used in IHC (Paraffin sections) (IHC-P). GTX113203
Anti-GPI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113203
Anti-GPI antibody used in Western Blot (WB). GTX113203
Anti-GPI antibody used in Western Blot (WB). GTX113203
Anti-GPI antibody used in IHC (Frozen sections) (IHC-Fr). GTX113203
Anti-GPI antibody used in IHC (Paraffin sections) (IHC-P). GTX113203
Anti-GPI antibody used in Western Blot (WB). GTX113203

Cat. No. GTX113203

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all GPI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

H1299 , Raji , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037119

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucose-6-phosphate isomerase , AMF , GNPI , NLK , PGI , PHI , SA-36 , SA36

Cellular Localization

Cytoplasm , Secreted

Background

This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GPI antibody used in Western Blot (WB). GTX113203

GTX113203 WB Image

KO/KD

Wild-type (WT) and GPI knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPI antibody (GTX113203) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GPI antibody used in Western Blot (WB). GTX113203

GTX113203 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Raji
7.5% SDS PAGE
GTX113203 diluted at 1:10000

Anti-GPI antibody used in IHC (Paraffin sections) (IHC-P). GTX113203

GTX113203 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GPI(GTX113203) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113203

GTX113203 ICC/IF Image

GPI antibody detects GPI protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: GPI protein stained by GPI antibody (GTX113203) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-GPI antibody used in Western Blot (WB). GTX113203

GTX113203 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPI antibody (GTX113203) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GPI antibody used in Western Blot (WB). GTX113203

GTX113203 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX113203 diluted at 1:1000

Anti-GPI antibody used in IHC (Frozen sections) (IHC-Fr). GTX113203

GTX113203 IHC-Fr Image

Immunohistochemical analysis of frozen sections of zebrafish retina, using GPI antibody (GTX113203) at 1:2000 dilution.

Anti-GPI antibody used in IHC (Paraffin sections) (IHC-P). GTX113203

GTX113203 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using GPI antibody (GTX113203) at 1:300 dilution.

Anti-GPI antibody used in Western Blot (WB). GTX113203

GTX113203 WB Image

The data was published in the journal Viruses in 2018. PMID: 29518929

REFERENCE

Application Reference

Ma M et al. Cell Commun Signal 2023; 21 (1) : 7 LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch myofibers.
Kavanagh Williamson M et al. Viruses 2018; 10 (3) Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1.
Application : WB
Reactivity : Human
Wu YH et al. PLoS One 2013; 8 (8) : e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169