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GPI antibody

KO/KD
Anti-GPI antibody used in Western Blot (WB). GTX113203
Anti-GPI antibody used in Western Blot (WB). GTX113203
Anti-GPI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113203
Anti-GPI antibody used in IHC (Paraffin sections) (IHC-P). GTX113203
Anti-GPI antibody used in Western Blot (WB). GTX113203

Cat No. GTX113203

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($379),
25 μl ($169)
See all GPI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

H1299 , Raji

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037119

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glucose-6-phosphate isomerase , AMF , GNPI , NLK , PGI , PHI , SA-36 , SA36

Cellular Localization

Cytoplasm , Secreted

Background

This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GPI antibody used in Western Blot (WB). GTX113203

GTX113203 WB Image

KO/KD

Wild-type (WT) and GPI knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPI antibody (GTX113203) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GPI antibody used in Western Blot (WB). GTX113203

GTX113203 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPI antibody (GTX113203) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GPI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113203

GTX113203 ICC/IF Image

GPI antibody detects GPI protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: GPI protein stained by GPI antibody (GTX113203) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-GPI antibody used in IHC (Paraffin sections) (IHC-P). GTX113203

GTX113203 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GPI(GTX113203) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPI antibody used in Western Blot (WB). GTX113203

GTX113203 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Raji
7.5% SDS PAGE
GTX113203 diluted at 1:10000

REFERENCE

Application Reference

Kavanagh Williamson M et al. Viruses 2018; 10 (3) Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1.
Application : WB
Reactivity : Human
Wu YH et al. PLoS One 2013; 8 (8) : e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.
Application : WB
Reactivity : Human
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REVIEW

GPI antibody Cat No. GTX113203

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-GPI antibody used in Western Blot (WB). GTX113203
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Viruses in 2018. PMID: 29518929
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169