APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:20000 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.57 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human GPX4 .
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutathione peroxidase 4 , GPx-4 , GSHPx-4 , MCSP , PHGPx , SMDS , snGPx , snPHGPx
Background
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016]
Database
Research Area
DATA IMAGES
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GTX03194 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX4 antibody [GT1282] (GTX03194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX03194 IP Image
IP analysis of Jurkat whole cell lysate using GTX03194 GPX4 antibody [GT1282].
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GTX03194 WB Image
WB analysis of various samples using GTX03194 GPX4 antibody [GT1282]. Dilution : 1:1000 Loading : 25μg per lane
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REFERENCE
There are currently no references for GPX4 antibody [GT1282] (GTX03194). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GPX4 antibody [GT1282] (GTX03194). Be the first to share your experience with this product.