50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Store at -80ºC. Product is stable for at least 6-12 months.
0.1mg/ml(Please refer to the vial label for the specific concentration.)
Baculovirus (Sf9 insect cells)
Purity was assessed by SDS-PAGE (≥95%) and by HPLC.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
G proteincoupled receptor kinase5, GRK5, G proteincoupled receptor kinase 5, G protein-coupled receptor kinase 5, GRK
G protein-coupled receptor kinases (GRKs) play an important role in phosphorylating and regulating the activity of a variety of G protein-coupled receptors. Haribabu and Snyderman (1) identified GRK5 and GRK6. GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). Bullrich (2) used a rodent-human hybrid panel to map 2 newly identified members of the GRK family: GPRK5 and GPRK6 to 10q24-qter and 5q35, respectively. Desensitization of G protein-coupled receptors regulates the number of polymorphonuclear leukocytes (PMNs), as well as their motility and ability to stop upon contact with pathogens or target cells, and this desensitization is mediated by GRKs (3). MIP2 induces GRK2 and GRK5 expression in PMNs through PI3KG signaling. However, lipopolysaccharide (LPS), acting through TLR4 signaling, mediated through MEK1 /MEK2, transcriptionally downregulates expression of GRK2 and GRK5 in response to MIP2, which decreases chemokine receptor desensitization and markedly augments PMN migration. Thus, LPS-activated TLR4 signaling regulates PMN migration by modulating the expression of chemokine receptors in a GRK2- and GRK5-dependent manner.