Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody (GTX133372) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody (GTX133372) diluted at 1:1000.

GSK3 beta antibody detects GSK3 beta protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSK3 beta stained by GSK3 beta antibody (GTX133372) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker diluted at 1:200.
Blue: Hoechst 33342 staining.

Wild-type (WT) and GSK3 beta knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody (GTX133372) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.