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GST tag antibody

Anti-GST tag antibody used in Western Blot (WB). GTX26633
Anti-GST tag antibody used in Immunoprecipitation (IP). GTX26633
Anti-GST tag antibody used in ELISA (ELISA). GTX26633
Anti-GST tag antibody used in Western Blot (WB). GTX26633
Anti-GST tag antibody used in Western Blot (WB). GTX26633
Anti-GST tag antibody used in Immunoprecipitation (IP). GTX26633

Cat. No. GTX26633

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA, IHC

Reactivity

 Species independent
Package
100 μg ($399)
See all GST tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IP Assay dependent
ELISA 1:20000-1:50000
IHC 1:1:1000-1:10000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Schistosoma japonicum

Immunogen

Glutathione-S-Transferase collected from Schistosoma japonicum

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

The GST gene is used in many expression cloning vectors and provides an additional means of fusion protein detection. The antibody is also useful for quantitative measurements of fusion proteins, or when linked to a solid phase, as a means of purification.

Research Area

DATA IMAGES

Anti-GST tag antibody used in Western Blot (WB). GTX26633

GTX26633 WB Image

GeneTex anti-GST polyclonal antibody (GTX26633) in western blot shows detection of recombinant GST (indicated by band at ~ 28 kDa). The SDS-PAGE contained approximately 0.2 μg of rGST loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was transferred to nitrocellulose and blocked with “Blocking Buffer for Fluorescent Western Blotting” in TBS for 1h at RT. The membrane was probed with anti-GST antibody at a 1:2,000 dilution in blocking reagent, overnight at 4º C. For detection DyLight™800 conjugated Donkey-a-Goat IgG was used at a 1:20,000 dilution (in blocking reagent) for 30 min at 25º C.

Anti-GST tag antibody used in Immunoprecipitation (IP). GTX26633

GTX26633 IP Image

Immunoprecipitation of anti-GST antibody. Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 μg per lane. Primary antibody: anti-GST 1:400 for overnight at 4ºC. Secondary antibody: IRDye800™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC.

Anti-GST tag antibody used in ELISA (ELISA). GTX26633

GTX26633 ELISA Image

ELISA analysis of recombinant GST protein using serially diluted GTX26633 GST tag antibody.
Coating : 1 μg

Anti-GST tag antibody used in Western Blot (WB). GTX26633

GTX26633 WB Image

WB analysis of various samples using GTX26633 GST tag antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : CHO-K1 whole cell lysate
Lane 4 : MDA-MB-231 whole cell lysate
Lane 5 : A431 whole cell lysate
Lane 6 : Jurkat whole cell lysate
Lane 7 : NIH-3T3 whole cell lysate
Lane 8 : E. coli HCP
Lane 9 : Flag-tag protein
Lane 10 : RFP
Lane 11 : GFP
Lane 12 : GST protein
Lane 13 : MBP
Dilution : 1 μg/mL

Anti-GST tag antibody used in Western Blot (WB). GTX26633

GTX26633 WB Image

WB analysis of recombinant GST proteins using GTX26633 GST tag antibody.
Lane 1 : Protein ladder
Lane 2 : 0.1 μg
Lane 3 : 0.05 μg
Lane 4 : 0.02 μg
Lane 5 : Mock
Dilution : 1 μg/mL

Anti-GST tag antibody used in Immunoprecipitation (IP). GTX26633

GTX26633 IP Image

IP analysis of recombinant proteins using GTX26633 GST tag antibody.
Lane 1 : Recombinant GST-Pin1 protein
Lane 2 : Recombinant GST-CDK4 protein
Lane 3 : Recombinant GST-Pin1 and GST-CDK4 protein
Loading : 25 μg

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399