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GST tag antibody

Anti-GST tag antibody used in Immunoprecipitation (IP). GTX26633
Anti-GST tag antibody used in Western Blot (WB). GTX26633

Cat No. GTX26633

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA, IHC
Package
100 μg ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody recognizes protein that contain the GST epitope tag.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride containing 0.01% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Glutathione-S-Transferase [Schistosoma japonicum]

Purification

Affinity purified with antigen.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

The GST gene is used in many expression cloning vectors and provides an additional means of fusion protein detection. The antibody is also useful for quantitative measurements of fusion proteins, or when linked to a solid phase, as a means of purification.

Research Area

DATA IMAGES

Anti-GST tag antibody used in Immunoprecipitation (IP). GTX26633

GTX26633 IP Image

Immunoprecipitation of anti-GST antibody. Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 μg per lane. Primary antibody: anti-GST 1:400 for overnight at 4ºC. Secondary antibody: IRDye800™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC.

Anti-GST tag antibody used in Western Blot (WB). GTX26633

GTX26633 WB Image

GeneTex anti-GST polyclonal antibody (GTX26633) in western blot shows detection of recombinant GST (indicated by band at ~ 28 kDa). The SDS-PAGE contained approximately 0.2 μg of rGST loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was transferred to nitrocellulose and blocked with “Blocking Buffer for Fluorescent Western Blotting” in TBS for 1h at RT. The membrane was probed with anti-GST antibody at a 1:2,000 dilution in blocking reagent, overnight at 4º C. For detection DyLight™800 conjugated Donkey-a-Goat IgG was used at a 1:20,000 dilution (in blocking reagent) for 30 min at 25º C.

REFERENCE

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Package List Price ($)
$ 169