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Galectin 3 antibody [HL2020]

Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921
Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921
Anti-Galectin 3 antibody [HL2020] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637921
Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921
Anti-Galectin 3 antibody [HL2020] used in IHC (Paraffin sections) (IHC-P). GTX637921
Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921
Anti-Galectin 3 antibody [HL2020] used in IHC (Paraffin sections) (IHC-P). GTX637921

Cat. No. GTX637921

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2020

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Galectin 3

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921

GTX637921 WB Image

Wild-type (WT) and LGALS3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921

GTX637921 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Galectin 3 antibody [HL2020] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637921

GTX637921 ICC/IF Image

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921

GTX637921 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [HL2020] used in IHC (Paraffin sections) (IHC-P). GTX637921

GTX637921 IHC-P Image

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921

GTX637921 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [HL2020] used in IHC (Paraffin sections) (IHC-P). GTX637921

GTX637921 IHC-P Image

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cell membrane and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat stomach.
Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

Galectin 3 antibody [HL2020] Cat. No. GTX637921

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : Calu-3 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 16Hr
Package List Price ($)
$ 399
$ 169
Custom Conjugations