Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Galectin 4 antibody (GTX114527) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Galectin4(GTX114527) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: LGALS4 transfected 293T lysate
10% SDS PAGE
GTX114527 diluted at 1:5000