Gephyrin antibody [N2C1], Internal detects Gephyrin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Gephyrin antibody [N2C1], Internal detects Gephyrin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Immunoprecipitation of Gephyrin protein from A431 whole cell extracts using 5 μg of Gephyrin antibody [N2C1], Internal (GTX109734).
Western blot analysis was performed using Gephyrin antibody [N2C1], Internal (GTX109734).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX109734 diluted at 1:1000

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Gephyrin antibody [N2C1], Internal detects Gephyrin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Gephyrin stained by Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min