Human giantin is detected by immunocytochemistry using anti-Giantin, mAb (rec.) (TA10) (ATTO 488). Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with using anti-Giantin, mAb (rec.) (TA10) (ATTO 488) (1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are mounted in Moewiol. Nuclei are stained with DAPI.