Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody (GTX124274) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Gli1 antibody detects Gli1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: RMS-13 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Gli1 stained by Gli1 antibody (GTX124274) diluted at 1:5000.

The data was published in the journal Cancer Cell Int in 2017. PMID: 29225516

The data was published in the journal Cancer Cell Int in 2017. PMID: 29225516

The data was published in the journal Cancer Cell Int in 2017. PMID: 29225516