GeneTex
United States (US)

Glucagon receptor antibody [N1N2], N-term

GTX100384_WB_19011506_451.jpg
GTX100384_41605_WB_19011506_642.jpg
GTX100384_IHC_19011506_433.jpg

Cat No. GTX100384

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , H1299 , HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Glucagon Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ97182 antibody, GGR antibody, MGC138246 antibody, GCGR antibody, GL-R antibody, glucagon receptor antibody

Background

The physiologic effects of glucagon (GCG; MIM 138030) are mediated through the glucagon receptor, a member of the superfamily of receptors characterized by a 7-transmembrane domain structure and by their coupling via GTP-binding proteins (G proteins) to adenyl cyclase.[supplied by OMIM]

Database

DATA IMAGES
GTX100384_WB_19011506_451.jpg

GTX100384 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
10% SDS PAGE
GTX100384 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100384_41605_WB_19011506_642.jpg

GTX100384 WB Image

Glucagon Receptor antibody detects Glucagon Receptor protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glucagon Receptor antibody (GTX100384) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100384_IHC_19011506_433.jpg

GTX100384 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using Glucagon Receptor(GTX100384) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169