APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Glucocorticoid Receptor. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909981
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear receptor subfamily 3 group C member 1 , GCCR , GCR , GCRST , GR , GRL
Cellular Localization
Nucleus,Mitochondrion,Cytoplasm
Background
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
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GTX636422 WB Image
Wild-type (WT) and Glucocorticoid Receptor knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [HL1151] (GTX636422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636422 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [HL1151] (GTX636422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636422 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [HL1151] (GTX636422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636422 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [HL1151] (GTX636422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636422 ICC/IF Image
Glucocorticoid Receptor antibody [HL1151] detects Glucocorticoid Receptor protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody [HL1151] (GTX636422) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX636422 IHC-P Image
Glucocorticoid Receptor antibody [HL1151] detects Glucocorticoid Receptor protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody [HL1151] (GTX636422) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636422 IHC-P Image
Glucocorticoid Receptor antibody [HL1151] detects Glucocorticoid Receptor protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody [HL1151] (GTX636422) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636422 IHC-P Image
Glucocorticoid Receptor antibody [HL1151] detects Glucocorticoid Receptor protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat muscle.Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody [HL1151] (GTX636422) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for Glucocorticoid Receptor antibody [HL1151] (GTX636422). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Glucocorticoid Receptor antibody [HL1151] (GTX636422). Be the first to share your experience with this product.