Glutamine synthetase antibody

Name: Glutamine synthetase antibody
Brand: GeneTex
SKU: GTX109121
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

Anti-Glutamine synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109121

Anti-Glutamine synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX109121

Anti-Glutamine synthetase antibody used in Immunoprecipitation (IP). GTX109121

Anti-Glutamine synthetase antibody used in IHC (Frozen sections) (IHC-Fr). GTX109121

Cat No. GTX109121

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC
Reactivity	Human, Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

See all Glutamine synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IP	1:100-1:500
IHC	Assay dependent

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , mouse eye , rat brain

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Glutamine synthetase Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59

Cellular Localization

Cytoplasm

Background

Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

Database

Gene ID: 2752 GLUL
UniProt: P15104 GLUL

Research Area

DATA IMAGES

GTX109121 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109121 ICC/IF Image

Glutamine synthetase antibody detects Glutamine synthetase protein at astrocytes by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109121 IHC-P Image

Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.
Sample:Paraffin-embedded adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109121 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft , using Glutamine Synthetase (GTX109121) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109121 IP Image

Immunoprecipitation of Glutamine synthetase protein from IMR32 whole cell extracts using 5 μg of Glutamine synthetase antibody (GTX109121).
Western blot analysis was performed using Glutamine synthetase antibody (GTX109121).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109121 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109121 IHC-Fr Image

Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109121 WB Image

Glutamine synthetase antibody detects Glutamine synthetase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109121 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.

GTX109121 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:20000.

GTX109121 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.

REFERENCE

Application Reference

Mundorf A et al. Psychiatry Res 2019; 281 : 112544 Lithium and glutamine synthetase: Protective effects following stress.

Application : IHC

Reactivity : Mouse

Pronin A et al. Front Mol Neurosci 2019; 12 : 36 Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury.

Nakashima K et al. Sci Rep 2019; 9 (1) : 14818 A spatial similarity of stereochemical environments formed by amino acid residues defines a common epitope of two non-homologous proteins.

Application : ICC/IF

Reactivity : Human

Kamei Y et al. Am J Physiol Endocrinol Metab 2014; 307 (6) : E485-93 FOXO1 activates glutamine synthetase gene in mouse skeletal muscles through a region downstream of 3'-UTR: possible contribution to ammonia detoxification.

Submit a Reference

REVIEW