APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , mouse eye , rat brain
Predict Reactivity
Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human Glutamine synthetase Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950421
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59
Cellular Localization
Cytoplasm
Background
Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX109121 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109121 ICC/IF Image
Glutamine synthetase antibody detects Glutamine synthetase protein at astrocytes by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX109121 IHC-P Image
Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.
Sample:Paraffin-embedded adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX109121 IHC-P Image
Immunohistochemical analysis of paraffin-embedded H441 xenograft , using Glutamine Synthetase (GTX109121) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109121 IP Image
Immunoprecipitation of Glutamine synthetase protein from IMR32 whole cell extracts using 5 μg of Glutamine synthetase antibody (GTX109121).
Western blot analysis was performed using Glutamine synthetase antibody (GTX109121).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX109121 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
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GTX109121 WB Image
Whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109121 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.
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GTX109121 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.
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GTX109121 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.
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GTX109121 IHC-Fr Image
Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
REVIEW
Glutamine synthetase antibody
Cat No. GTX109121
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Rating
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( Average 5 based on 7 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 2 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 5 based on 1 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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IHC (Frozen sections)(IHC-Fr)
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( Average 5 based on 3 users reviews)
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Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Front Physiol in 2020.
PMID: 32425814
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2019.
PMID: 31616018
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
ICC/IF
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2019.
PMID: 31616018
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-Fr
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Front Mol Neurosci in 2019.
PMID: 30930743
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-Fr
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Front Mol Neurosci in 2019.
PMID: 30930743
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-Fr
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Front Mol Neurosci in 2019.
PMID: 30930743
Date :
Anonymous submitted on 25-Feb-2020
Application Tested :
IHC-P
Sample Species :
Hu
Sample :
Brain tissue
Blocking :
25°C, 1Hr
Permeabilization /Antigen retrieval :
1%BSA, 15% goat serum/Citrate buffer
Primary Antibody :
1:500, 4°C, 18Hr
Lane Description :
Red: Glutamine synthetase, Green: EAAT2, Blue: DAPI
![Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363](/upload/website/prouct_img/small/GTX635363/GTX635363_20200217_IHC-Fr_M_competitor_20021701_920.jpg "Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363")
![Anti-SOX2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101507](/upload/website/prouct_img/small/GTX101507/GTX101507_42046_20150814_IHC-P_M_19011506_157.jpg "Anti-SOX2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101507")
