GeneTex
United States (US)

Glutamine synthetase antibody

KO/KD Overexpression
GTX109121_41626_20160630_WB_shRNA_watermark_19011507_366.jpg
GTX109121_41626_20170727_IFA_R_19011507_803.jpg
GTX109121_41745_20170214_IHC-P_19011507_544.jpg
GTX109121_IHC_19011507_518.jpg
GTX109121_41647_20160901_WB_R_brain_19011507_319.jpg
GTX109121_41647_20160825_WB_M_eye_19011507_883.jpg
GTX109121_41626_20150618_IP_19011507_608.jpg
GTX109121_41647_20181005_WB_B_19011507_585.jpg
GTX109121_41647_20160901_WB_M_brain_19011507_417.jpg
GTX109121_41626_20170531_IHC-Fr_M_19011507_344.jpg
GTX109121_41626_20150320_WB_19011507_665.jpg

Cat No. GTX109121

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , mouse eye , rat brain

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Glutamine synthetase Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GLNS antibody, GS antibody, PIG43 antibody, PIG59 antibody, GLUL antibody, cell proliferation-inducing protein 59 antibody, glutamate--ammonia ligase antibody, glutamine synthetase antibody, proliferation-inducing protein 43 antibody, glutamate decarboxylase antibody, glutamine synthase antibody, glutamate-ammonia ligase antibody

Cellular Localization

Cytoplasm

Background

Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX109121_41626_20160630_WB_shRNA_watermark_19011507_366.jpg

GTX109121 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109121_41626_20170727_IFA_R_19011507_803.jpg

GTX109121 ICC/IF Image

Glutamine synthetase antibody detects Glutamine synthetase protein at astrocytes by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109121_41745_20170214_IHC-P_19011507_544.jpg

GTX109121 IHC-P Image

Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.
Sample:Paraffin-embedded adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109121_IHC_19011507_518.jpg

GTX109121 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft , using Glutamine Synthetase (GTX109121) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109121_41647_20160901_WB_R_brain_19011507_319.jpg

GTX109121 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.

GTX109121_41647_20160825_WB_M_eye_19011507_883.jpg

GTX109121 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:20000.

GTX109121_41626_20150618_IP_19011507_608.jpg

GTX109121 IP Image

Immunoprecipitation of Glutamine synthetase protein from IMR32 whole cell extracts using 5 μg of Glutamine synthetase antibody (GTX109121).
Western blot analysis was performed using Glutamine synthetase antibody (GTX109121).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109121_41647_20181005_WB_B_19011507_585.jpg

GTX109121 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109121_41647_20160901_WB_M_brain_19011507_417.jpg

GTX109121 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.

GTX109121_41626_20170531_IHC-Fr_M_19011507_344.jpg

GTX109121 IHC-Fr Image

Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109121_41626_20150320_WB_19011507_665.jpg

GTX109121 WB Image

Glutamine synthetase antibody detects Glutamine synthetase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169