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Glycogen synthase 1 (phospho Ser641) antibody

Anti-Glycogen synthase 1 (phospho Ser641) antibody used in Western Blot (WB). GTX22479
Anti-Glycogen synthase 1 (phospho Ser641) antibody used in IHC (Paraffin sections) (IHC-P). GTX22479

Cat. No. GTX22479

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all Glycogen synthase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:10000
IHC-P 1:1000 - 1:5000
ELISA 1:20000 - 1:60000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Product Note

Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Muscle Glycogen Synthase phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - AQGYRYPRPA(pS)VP (aa 631-642).

Purification

Purified by antigen-affinity chromatography.
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed agains

Conjugation

Unconjugated

RRID

AB_374324

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase 1 , GSY , GYS

Background

Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at Ser 640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.

Database

Research Area

DATA IMAGES

Anti-Glycogen synthase 1 (phospho Ser641) antibody used in Western Blot (WB). GTX22479

GTX22479 WB Image

Affinity Purified Phospho-specific antibody to human muscle Glycogen Synthase at pS640 (GTX22479) was used at a 1:1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 μM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system.

Anti-Glycogen synthase 1 (phospho Ser641) antibody used in IHC (Paraffin sections) (IHC-P). GTX22479

GTX22479 IHC-P Image

Immunohistochemistry with Anti-Glycogen Synthase at pS641antibody (GTX22479) Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody: 5 μg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

REFERENCE

Application Reference

Brull A et al. J Physiol 2015; 593 (12) : 2693-706 Phenotype consequences of myophosphorylase dysfunction: insights from the McArdle mouse model.
Application : WB
Reactivity : Mouse
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399