Glypican-3 antibody [GT2473]

Name: Glypican-3 antibody [GT2473]
Brand: GeneTex
SKU: GTX633410
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Glypican-3 antibody [GT2473] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633410

Anti-Glypican-3 antibody [GT2473] used in IHC (Paraffin sections) (IHC-P). GTX633410

Anti-Glypican-3 antibody [GT2473] used in Western Blot (WB). GTX633410

Cat. No. GTX633410

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	GT2473
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Glypican-3 products

PRODUCT

Summary

Glypican-3 (GPC3) is a glycoprotein attached to the cell membrane. It has a predicted molecular weight of 66 kDa. GPC3 overexpression is found in human hepatocellular carcinoma (HCC), but not in healthy adult liver. Studies have shown GPC3 to be a reliable serological and immunohistochemical marker for HCC diagnosis, and a potential target for immunotherapies.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888342

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-3: Secreted , extracellular space

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. [provided by RefSeq]

Database

Gene ID: 2719 GPC3
UniProt: P51654 GPC3

Research Area

DATA IMAGES

GTX633410 ICC/IF Image

Glypican-3 antibody [GT2473] detects Glypican-3 protein at cell membrane by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glypican-3 protein stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:250.
Blue: Hoechst 33342 staining.

GTX633410 IHC-P Image

Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Glypican-3 stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX633410 IHC-P Image

Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human liver cancer.
Glypican-3 stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:100.
Antigen Retrieval: Citrate buffer, 20 min

GTX633410 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Xue Y et al. Am J Pathol 2018; (Epub) Hepatitis C Virus Mimics Effects of Glypican-3 on CD81 and Promotes Development of Hepatocellular Carcinomas via Activation of Hippo Pathway in Hepatocytes.

Application : WB

Reactivity : Human

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