PRODUCT
Summary
Glypican-3 (GPC3) is a glycoprotein attached to the cell membrane. It has a predicted molecular weight of 66 kDa. GPC3 overexpression is found in human hepatocellular carcinoma (HCC), but not in healthy adult liver. Studies have shown GPC3 to be a reliable serological and immunohistochemical marker for HCC diagnosis, and a potential target for immunotherapies.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.4 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888342
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1
Cellular Localization
Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-3: Secreted , extracellular space
Background
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX633410 ICC/IF Image
Glypican-3 antibody [GT2473] detects Glypican-3 protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glypican-3 protein stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:250. Blue: Hoechst 33342 staining.
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GTX633410 IHC-P Image
Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic cancer.Glypican-3 stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633410 IHC-P Image
Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human liver cancer.Glypican-3 stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:100.Antigen Retrieval: Citrate buffer, 20 min
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GTX633410 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
Glypican-3 antibody [GT2473]
Cat. No. GTX633410
Rating
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( Average 5 based on 1 users reviews)
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Application
IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 21-Oct-2020
Application Tested :
IHC-P
Sample Species :
Hu
Sample :
liver cancer tissue
Fixation :
Citrate buffer, 0.3°C
Primary Antibody :
1:100, 4°C, 16Hr