PRODUCT
Summary
Glypican-3 (GPC3) is a glycoprotein attached to the cell membrane. It has a predicted molecular weight of 66 kDa. GPC3 overexpression is found in human hepatocellular carcinoma (HCC), but not in healthy adult liver. Studies have shown GPC3 to be a reliable serological and immunohistochemical marker for HCC diagnosis, and a potential target for immunotherapies.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.4 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888342
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1
Cellular Localization
Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-3: Secreted , extracellular space
Background
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX633410 ICC/IF Image
Glypican-3 antibody [GT2473] detects Glypican-3 protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glypican-3 protein stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:250. Blue: Hoechst 33342 staining.
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GTX633410 IHC-P Image
Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic cancer.Glypican-3 stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633410 IHC-P Image
Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human liver cancer.Glypican-3 stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:100.Antigen Retrieval: Citrate buffer, 20 min
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GTX633410 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX633410 WB Image
Unboiled various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
REVIEW
Glypican-3 antibody [GT2473]
Cat. No. GTX633410
Rating
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( Average 5 based on 1 users reviews)
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Application
IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 21-Oct-2020
Application Tested :
IHC-P
Sample Species :
Hu
Sample :
liver cancer tissue
Fixation :
Citrate buffer, 0.3°C
Primary Antibody :
1:100, 4°C, 16Hr