Glypican-3 antibody [GT2473]

Name: Glypican-3 antibody [GT2473]
Brand: GeneTex
SKU: GTX633410
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-Glypican-3 antibody [GT2473] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633410

Anti-Glypican-3 antibody [GT2473] used in IHC (Paraffin sections) (IHC-P). GTX633410

Anti-Glypican-3 antibody [GT2473] used in Western Blot (WB). GTX633410

Cat No. GTX633410

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	GT2473
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($329), 25 μl ($169)

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PRODUCT

Summary

Glypican-3 (GPC3) is a glycoprotein attached to the cell membrane. It has a predicted molecular weight of 66 kDa. GPC3 overexpression is found in human hepatocellular carcinoma (HCC), but not in healthy adult liver. Studies have shown GPC3 to be a reliable serological and immunohistochemical marker for HCC diagnosis, and a potential target for immunotherapies.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

HepG2 , HepG2 (24 μg/ml Tunicamycin treatment for 16 hr)

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888342

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-3: Secreted , extracellular space

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. [provided by RefSeq]

Database

Gene ID: 2719 GPC3
UniProt: P51654 GPC3

Research Area

DATA IMAGES

GTX633410 ICC/IF Image

Glypican-3 antibody [GT2473] detects Glypican-3 protein at cell membrane by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glypican-3 protein stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:250.
Blue: Hoechst 33342 staining.

GTX633410 IHC-P Image

Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Glypican-3 stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX633410 IHC-P Image

Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human liver cancer.
Glypican-3 stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:100.
Antigen Retrieval: Citrate buffer, 20 min

GTX633410 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Xue Y et al. Am J Pathol 2018; (Epub) Hepatitis C Virus Mimics Effects of Glypican-3 on CD81 and Promotes Development of Hepatocellular Carcinomas via Activation of Hippo Pathway in Hepatocytes.

Application : WB

Reactivity : Human

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