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H-Ras antibody

Comparison
Anti-H-Ras antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116041
Anti-H-Ras antibody used in IHC (Paraffin sections) (IHC-P). GTX116041
Anti-H-Ras antibody used in IHC (Paraffin sections) (IHC-P). GTX116041
Anti-H-Ras antibody used in Western Blot (WB). GTX116041
Anti-H-Ras antibody used in Western Blot (WB). GTX116041
Anti-H-Ras antibody used in Western Blot (WB). GTX116041
Anti-H-Ras antibody used in Western Blot (WB). GTX116041

Cat No. GTX116041

Reference 5
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all H-Ras products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , HRAS-transfected 293T , *RFL6 , *PLC5Rat2 , *RFL6 , *PLC5

Specificity/Sensitivity

This antibody is specific for H-Ras protein, and it does not cross react with human N-Ras or K-Ras protein.

Predict Reactivity

Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human H-Ras. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HRas proto-oncogene, GTPase , C-BAS/HAS , C-H-RAS , C-HA-RAS1 , CTLO , H-RASIDX , HAMSV , HRAS1 , KRAS , KRAS2 , Ki-Ras , RASH1 , RASK2 , c-K-ras , c-Ki-ras , p21ras

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side , Golgi apparatus membrane; Lipid-anchor

Background

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-H-Ras antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116041

GTX116041 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-RAS (GTX116041) antibody at 1:200 dilution.

Anti-H-Ras antibody used in IHC (Paraffin sections) (IHC-P). GTX116041

GTX116041 IHC-P Image

H-RAS antibody detects H-RAS protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
H-RAS antibody (GTX116041) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-H-Ras antibody used in IHC (Paraffin sections) (IHC-P). GTX116041

GTX116041 IHC-P Image

H-RAS antibody detects H-RAS protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
H-RAS antibody (GTX116041) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-H-Ras antibody used in Western Blot (WB). GTX116041

GTX116041 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with H-RAS antibody (GTX116041) diluted at 1:500 and competitor's antibody (SC-520) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-H-Ras antibody used in Western Blot (WB). GTX116041

GTX116041 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody (GTX116041) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-H-Ras antibody used in Western Blot (WB). GTX116041

GTX116041 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody (GTX116041) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-H-Ras antibody used in Western Blot (WB). GTX116041

GTX116041 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody (GTX116041) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Gao P et al. Breast Cancer Res 2018; 20 (1) : 112 nogo-b receptor increases the resistance to * in estrogen receptor-positive breast cancer cells.
Application : IP
Reactivity : Human
Sugita S et al. Int J Oncol 2018; (Epub) HRAS as a potential therapeutic target of salirasib RAS inhibitor in bladder cancer.
Reactivity : Human
Sarin N et al. Int J Mol Sci 2018; 19 (3) key players of * resistance: towards a systems pharmacology approach.
Application : WB
Reactivity : Human
Brandt LP et al. PLoS One 2017; 12 (10) : e0186102 Oncogenic HrasG12V expression plus knockdown of Cdkn2a using ecotropic lentiviral vectors induces high-grade endometrial stromal sarcoma.
Application : IHC-P
Reactivity : Mouse
Tsuboi M et al. J Biol Chem 2017; (Epub) The Transcription Factor HOXB7 Regulates ERK Kinase Activity and Thereby Stimulates the Motility and Invasiveness of Pancreatic Cancer Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

H-Ras antibody Cat No. GTX116041

Rating
( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 3 users reviews)
Anti-H-Ras antibody used in Western Blot (WB). GTX116041
Date : Anonymous submitted on 26-Feb-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : human H-Ras transfected 293T cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 0.3Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane1: 293T cells transfected with pBabe-human H-ras. Lane2: 293T cells transfected with pBabe-puro.
Anti-H-Ras antibody used in Western Blot (WB). GTX116041
Date : Anonymous submitted on 10-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : PLC5 cells
Amount used : 20μg
Blocking : 5% skim milk in PBST, 4°C, overnightHr
Primary Antibody : 1:2000, 25°C, 1Hr
Anti-H-Ras antibody used in Western Blot (WB). GTX116041
Date : Anonymous submitted on 13-May-2014
Score :
Application Tested : WB
Sample Species : Rat
Sample : RFL6 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:600, 4°C, 18Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169
Currently in production