GeneTex
United States (US)

H-RAS antibody

GTX116041_42823_20160420_WB_18121410_417.jpg
GTX116041_40135_WB_R_18121410_905.jpg
GTX116041_IFA_18121410_958.jpg
GTX116041_40135_20161124_WB_competitor_watermark_18121410_234.jpg
GTX116041_40135_20141009_IHC_M_18121410_529.jpg
GTX116041_40135_20141009_IHC_M_2_18121410_789.jpg
GTX116041_42823_20170427_WB_B_18121410_350.jpg

Cat No. GTX116041

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , HRAS-transfected 293T , *RFL6 , *PLC5Rat2 , *RFL6 , *PLC5

Specificity/Sensitivity

This antibody is specific for H-Ras protein, and it does not cross react with human N-Ras or K-Ras protein.

Predict Reactivity

Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human H-Ras. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hras Proto-Oncogene, Gtpase,C-Bas/Has,C-H-Ras,C-Ha-Ras1,Ctlo,H-Rasidx,Hamsv,Hras1,Rash1,P21Ras,Hras

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side , Golgi apparatus membrane; Lipid-anchor

Background

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX116041_42823_20160420_WB_18121410_417.jpg

GTX116041 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody (GTX116041) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116041_40135_WB_R_18121410_905.jpg

GTX116041 WB Image

H-Ras antibody detects HRAS protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12% SDS-PAGE
H-Ras antibody (GTX116041) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116041_IFA_18121410_958.jpg

GTX116041 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras (GTX116041) antibody at 1:200 dilution.

GTX116041_40135_20161124_WB_competitor_watermark_18121410_234.jpg

GTX116041 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with H-Ras antibody (GTX116041) diluted at 1:500 and competitor's antibody (SC-520) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX116041_40135_20141009_IHC_M_18121410_529.jpg

GTX116041 IHC-P Image

H-Ras antibody detects H-Ras protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
H-Ras antibody (GTX116041) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116041_40135_20141009_IHC_M_2_18121410_789.jpg

GTX116041 IHC-P Image

H-Ras antibody detects H-Ras protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
H-Ras antibody (GTX116041) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116041_42823_20170427_WB_B_18121410_350.jpg

GTX116041 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Myc-DDDDK-tagged H-Ras antibody (GTX116041) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

H-RAS antibody Cat No. GTX116041

Rating ( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.3 based on 3 users reviews)
Date : Anonymous submitted on 26-Feb-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : human H-Ras transfected 293T cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 0.3Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane1: 293T cells transfected with pBabe-human H-ras. Lane2: 293T cells transfected with pBabe-puro.
Date : Anonymous submitted on 10-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : PLC5 cells
Amount used : 20μg
Blocking : 5% skim milk in PBST, 4°C, overnightHr
Primary Antibody : 1:2000, 25°C, 1Hr
Date : Anonymous submitted on 13-May-2014
Score :
Application Tested : WB
Sample Species : Rat
Sample : RFL6 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:600, 4°C, 18Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169