APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , HRAS-transfected 293T , *RFL6 , *PLC5Rat2 , *RFL6 , *PLC5
Specificity/Sensitivity
This antibody is specific for H-Ras protein, and it does not cross react with human N-Ras or K-Ras protein.
Predict Reactivity
Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
3.47 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human H-Ras. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Hras Proto-Oncogene, Gtpase,C-Bas/Has,C-H-Ras,C-Ha-Ras1,Ctlo,H-Rasidx,Hamsv,Hras1,Rash1,P21Ras,Hras
Cellular Localization
Cell membrane; Lipid-anchor; Cytoplasmic side , Golgi apparatus membrane; Lipid-anchor
Background
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX116041 WB Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody (GTX116041) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX116041 WB Image
H-Ras antibody detects HRAS protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12% SDS-PAGE
H-Ras antibody (GTX116041) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX116041 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras (GTX116041) antibody at 1:200 dilution.
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GTX116041 WB Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with H-Ras antibody (GTX116041) diluted at 1:500 and competitor's antibody (SC-520) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX116041 IHC-P Image
H-Ras antibody detects H-Ras protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
H-Ras antibody (GTX116041) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX116041 IHC-P Image
H-Ras antibody detects H-Ras protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
H-Ras antibody (GTX116041) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX116041 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Myc-DDDDK-tagged H-Ras antibody (GTX116041) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
H-RAS antibody
Cat No. GTX116041
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Rating
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( Average 4.3 based on 3 users reviews)
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Application
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Western Blot(WB)
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( Average 4.3 based on 3 users reviews)
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Date :
Anonymous submitted on 26-Feb-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
human H-Ras transfected 293T cells
Amount used :
50μg
Blocking :
5% non-fat milk, 25°C, 0.3Hr
Primary Antibody :
1:1000, 4°C, overnightHr
Lane Description :
Lane1: 293T cells transfected with pBabe-human H-ras. Lane2: 293T cells transfected with pBabe-puro.
Date :
Anonymous submitted on 10-Nov-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
PLC5 cells
Amount used :
20μg
Blocking :
5% skim milk in PBST, 4°C, overnightHr
Primary Antibody :
1:2000, 25°C, 1Hr
Date :
Anonymous submitted on 13-May-2014
Application Tested :
WB
Sample Species :
Rat
Sample :
RFL6 cells
Amount used :
30μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:600, 4°C, 18Hr
