HA tag antibody [GT134] detects HA tag protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA tagged protein expressing HeLa cells
7.5 % SDS-PAGE
HA tag antibody [GT134] (GTX629540) dilution: 1:5000

HA tag antibody [GT134] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 μg of HA tag antibody [GT134] (GTX629540) 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT134] (GTX629540) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.