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HDAC2 antibody

Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109642
Anti-HDAC2 antibody used in Western Blot (WB). GTX109642
Anti-HDAC2 antibody used in Immunoprecipitation (IP). GTX109642
Anti-HDAC2 antibody used in ChIP assay (ChIP assay). GTX109642
Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX109642
Anti-HDAC2 antibody used in Western Blot (WB). GTX109642
Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

Cat No. GTX109642

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , C2C12 , Rat2 , *NB2a

Product Note

Knockdown/Knockout validation was supported by customer review data.

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950481

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone deacetylase 2 , HD2 , KDAC2 , RPD3 , YAF1

Cellular Localization

Nucleus

Background

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109642

GTX109642 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC2(GTX109642) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

GTX109642 WB Image

HDAC2 antibody detects HDAC2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC2 antibody (GTX109642) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC2 antibody used in Immunoprecipitation (IP). GTX109642

GTX109642 IP Image

HDAC2 antibody immunoprecipitates HDAC2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC2 protein by 2 μg of HDAC2 antibody (GTX109642) 7.5% SDS-PAGE The immunoprecipitated HDAC2 protein was detected by HDAC2 antibody (GTX109642) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HDAC2 antibody used in ChIP assay (ChIP assay). GTX109642

GTX109642 ChIP assay Image

HDAC2 antibody immunoprecipitates HDAC2 protein-DNA complex in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract
A. 5 μg preimmune rabbit IgG
B. 5 μg of HDAC2 antibody (GTX109642)
The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.

Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX109642

GTX109642 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using HDAC2(GTX109642) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

GTX109642 WB Image

HDAC2 antibody detects HDAC2 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
HDAC2 antibody (GTX109642) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

GTX109642 WB Image

HDAC2 antibody detects HDAC2 protein by western blot analysis.
A. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
HDAC2 antibody (GTX109642) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

HDAC2 antibody Cat No. GTX109642

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-May-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : NB2a cells
Amount used : 5μg
Blocking : 10% skim milk, 25°C, 1Hr
Primary Antibody : 1:200, 25°C, 1Hr
Lane Description : Lane 1:NB2a cells; Lane 2:knockout HDAC2 in NB2a cells; Lane 3:knockout HDAC1 in NB2a cells
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf