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HDAC2 antibody

KO/KD Orthogonal
Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX109642
Anti-HDAC2 antibody used in ChIP assay (ChIP assay). GTX109642
Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109642
Anti-HDAC2 antibody used in Immunoprecipitation (IP). GTX109642
Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109642
Anti-HDAC2 antibody used in Western Blot (WB). GTX109642
Anti-HDAC2 antibody used in Western Blot (WB). GTX109642
Anti-HDAC2 antibody used in Western Blot (WB). GTX109642
Anti-HDAC2 antibody used in Western Blot (WB). GTX109642
Anti-HDAC2 antibody used in Western Blot (WB). GTX109642
Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

Cat. No. GTX109642

Reference 25
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Xenopus tropicalis
Package
100 μl ($399),
25 μl ($169)
See all HDAC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

C2C12 , Rat2

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950481

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 2 , HD2 , KDAC2 , RPD3 , YAF1

Cellular Localization

Nucleus

Background

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX109642

GTX109642 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using HDAC2(GTX109642) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC2 antibody used in ChIP assay (ChIP assay). GTX109642

GTX109642 ChIP assay Image

HDAC2 antibody immunoprecipitates HDAC2 protein-DNA complex in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract
A. 5 μg preimmune rabbit IgG
B. 5 μg of HDAC2 antibody (GTX109642)
The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.

Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109642

GTX109642 ICC/IF Image

HDAC2 antibody detects HDAC2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC2 stained by HDAC2 antibody (GTX109642) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HDAC2 antibody used in Immunoprecipitation (IP). GTX109642

GTX109642 IP Image

HDAC2 antibody immunoprecipitates HDAC2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC2 protein by 2 μg of HDAC2 antibody (GTX109642) 7.5% SDS-PAGE The immunoprecipitated HDAC2 protein was detected by HDAC2 antibody (GTX109642) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109642

GTX109642 ICC/IF Image

HDAC2 antibody detects HDAC2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC2 stained by HDAC2 antibody (GTX109642) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

GTX109642 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC2 antibody (GTX109642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

GTX109642 WB Image

HDAC2 antibody detects HDAC2 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
HDAC2 antibody (GTX109642) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

GTX109642 WB Image

HDAC2 antibody detects HDAC2 protein by western blot analysis.
A. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
HDAC2 antibody (GTX109642) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

GTX109642 WB Image

KO/KD

The data was published in the journal Front Pharmacol in 2017.PMID: 29326587

Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

GTX109642 WB Image

KO/KD

The data was published in the journal Front Pharmacol in 2016.PMID: 27065869

Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

GTX109642 WB Image

Orthogonal

The data was published in the 2022 in Int J Mol Sci. PMID: 35163312

REFERENCE

Application Reference

K Ghos et al. J Biol Chem 2024; 300 (11) : 107848 Nerve injury augments Cacna2d1 transcription via CK2-mediated phosphorylation of the histone deacetylase HDAC2 in dorsal root ganglia
Cassandri M et al. Cell Death Discov 2024; 10 (1) : 351 HDAC3 genetic and pharmacologic inhibition radiosensitizes fusion positive rhabdomyosarcoma by promoting DNA double-strand breaks.
Islam A et al. Nucleic Acids Res 2024; 52 (5) : 2416-2433 Site-specific acetylation of polynucleotide kinase 3'-phosphatase regulates its distinct role in DNA repair pathways.
N Tapryal et al. Nat Commun 2023; 14 (1) : 8169 The DNA glycosylase NEIL2 is protective during SARS-CoV-2 infection
Application : WB
Chakraborty A et al. bioRxiv 2023; A glycolytic metabolite restores DNA repair activity of polynucleotide kinase 3'-phosphatase in polyglutamine (PolyQ) diseases.
Show More +
Submit a Reference

REVIEW

HDAC2 antibody Cat. No. GTX109642

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-HDAC2 antibody used in Western Blot (WB). GTX109642
Date : Anonymous submitted on 21-May-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : NB2a cells
Amount used : 5μg
Blocking : 10% skim milk, 25°C, 1Hr
Primary Antibody : 1:200, 25°C, 1Hr
Lane Description : Lane 1:NB2a cells; Lane 2:knockout HDAC2 in NB2a cells; Lane 3:knockout HDAC1 in NB2a cells
Submit a Review
Application Reference
K Ghos et al. J Biol Chem 2024; 300 (11):107848 Nerve injury augments Cacna2d1 transcription via CK2-mediated phosphorylation of the histone deacetylase HDAC2 in dorsal root ganglia
Cassandri M et al. Cell Death Discov 2024; 10 (1):351 HDAC3 genetic and pharmacologic inhibition radiosensitizes fusion positive rhabdomyosarcoma by promoting DNA double-strand breaks.
Islam A et al. Nucleic Acids Res 2024; 52 (5):2416-2433 Site-specific acetylation of polynucleotide kinase 3'-phosphatase regulates its distinct role in DNA repair pathways.
N Tapryal et al. Nat Commun 2023; 14 (1):8169 The DNA glycosylase NEIL2 is protective during SARS-CoV-2 infection
Application : WB
Chakraborty A et al. bioRxiv 2023; A glycolytic metabolite restores DNA repair activity of polynucleotide kinase 3'-phosphatase in polyglutamine (PolyQ) diseases.
Zhou JJ et al. Elife 2023; 12 Histone deacetylase 1 maintains lineage integrity through histone acetylome refinement during early embryogenesis.
Application : WB
Reactivity : Xenopus tropicalis
Yang HH et al. Int J Mol Sci 2022; 23 (3) Anti-Excitotoxic Effects of N-Butylidenephthalide Revealed by Chemically Insulted Purkinje Progenitor Cells Derived from SCA3 iPSCs.
Application : WB
Reactivity : Human
Duan Y et al. mBio 2022;:e0328721 Novel Function of Avian p53 in Binding to ALV-J LTR Contributes to Its Antiviral Roles.
Application : ICC/IF
Lin HY et al. Biomed Pharmacother 2022; 145:112431 Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A.
Application : WB
Reactivity : Human
Nisha Tapryal et al. J Biol Chem 2021; 296:100723 Intrapulmonary administration of purified NEIL2 abrogates NF-κB-mediated inflammation.
Application : WB
Reactivity : Mouse
Sayed IM et al. Cells 2020; 9 (9) The DNA Glycosylase NEIL2 Suppresses Fusobacterium-Infection-Induced Inflammation and DNA Damage in Colonic Epithelial Cells.
Application : WB
Reactivity : Mouse
Chen CY et al. Front Oncol 2020; 10:216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.
Application : WB
Reactivity : Human
Thang SK et al. J Agric Food Chem 2019; 67 (40):11119-11128 Xanthohumol Suppresses NPC1L1 Gene Expression through Downregulation of HNF-4α and Inhibits Cholesterol Uptake in Caco-2 Cells.
Application : WB
Reactivity : Human
Wei GJ et al. AAPS J 2019; 21 (5):86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
Application : WB
Reactivity : Human
Chang HY et al. Molecules 2019; 24 (3) The Cholesterol-Modulating Effect of Methanol Extract of Pigeon Pea (Cajanus cajan (L.) Millsp.) Leaves on Regulating LDLR and PCSK9 Expression in HepG2 Cells.
Huang D et al. Nat Immunol 2018; (Epub) NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.
Application : ChIP assay
Reactivity : Human
Gao WY et al. J Agric Food Chem 2018; 66 (24):6083-6093 Pinostrobin Inhibits Proprotein Convertase Subtilisin/Kexin-type 9 (PCSK9) Gene Expression through the Modulation of FoxO3a Protein in HepG2 Cells.
Application : WB
Reactivity : Human
Cencioni C et al. Nat Commun 2018; 9 (1):1281 Zeb1-Hdac2-eNOS circuitry identifies early cardiovascular precursors in naive mouse embryonic stem cells.
Application : IP
Reactivity : Mouse
Huang WT et al. Front Pharmacol 2017; 8:902 HDAC2 and HDAC5 Up-Regulations Modulate Survivin and miR-125a-5p Expressions and Promote Hormone Therapy Resistance in Estrogen Receptor Positive Breast Cancer Cells.
Chen SF et al. J Agric Food Chem 2017; (Epub) Xanthohumol suppresses Mylip/Idol gene expression and modulates LDLR abundance and activity in HepG2 cells.
Application : WB
Reactivity : Human
Tanegashima K et al. Genes Cells 2017; 22 (1):71-83 Epigenetic regulation of the glucose transporter gene Slc2a1 by β-hydroxybutyrate underlies preferential glucose supply to the brain of fasted mice.
Application : WB
Reactivity : Mouse
Lee JY et al. Front Pharmacol 2016; 7:81 Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells.
Application : WB
Reactivity : Human
Chen HC et al. PLoS One 2016; 11 (9):e0162414 Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells.
Application : WB
Reactivity : Human
Lee KH et al. Sci Rep 2014; 4:6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Application : WB
Reactivity : Human
Chen PY et al. PLoS One 2013; 8 (8):e71282 Down-regulation of the oncogene PTTG1 via the KLF6 tumor suppressor during induction of myeloid differentiation.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169