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HDAC3 antibody [C3], C-term

KO/KD
Anti-HDAC3 antibody [C3], C-term used in ChIP assay (ChIP assay). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109679
Anti-HDAC3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

Cat. No. GTX109679

Reference 8
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all HDAC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , U87-MG , SK-N-SH , IMR32 , SK-N-AS , 293T , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950482

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 3 , HD3 , KDAC3 , RPD3 , RPD3-2

Cellular Localization

Nucleus

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC3 antibody [C3], C-term used in ChIP assay (ChIP assay). GTX109679

GTX109679 ChIP assay Image

HDAC3 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of HDAC3 antibody (GTX109679) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Anti-HDAC3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX109679

GTX109679 IP Image

HDAC3 antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 μg of HDAC3 antibody (GTX109679) 10% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody (GTX109679) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109679

GTX109679 ICC/IF Image

HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

Sample(30 μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX109679 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109679

GTX109679 ICC/IF Image

HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:400.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-HDAC3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109679

GTX109679 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HDAC3(GTX109679) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

KO/KD

The data was published in the journal Front Pharmacol in 2016.PMID: 27065869

REFERENCE

Application Reference

Cassandri M et al. Cell Death Discov 2024; 10 (1) : 351 HDAC3 genetic and pharmacologic inhibition radiosensitizes fusion positive rhabdomyosarcoma by promoting DNA double-strand breaks.
Xu Y et al. Cell Biosci 2023; 13 (1) : 7 Activation of goblet cell Piezo1 alleviates mucus barrier damage in mice exposed to WAS by inhibiting H3K9me3 modification.
Application : WB
Reactivity : Human, Mouse
Richter HJ et al. Proc Natl Acad Sci U S A 2022; 119 (33) : e2205276119 Balanced control of thermogenesis by nuclear receptor corepressors in brown adipose tissue.
Chen CY et al. Front Oncol 2020; 10 : 216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.
Application : WB
Reactivity : Human
Huang D et al. Nat Immunol 2018; (Epub) NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.
Application : ChIP assay
Reactivity : Human
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REVIEW

HDAC3 antibody [C3], C-term Cat. No. GTX109679

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Date : Anonymous submitted on 06-Dec-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : FADU cells
Amount used : 15μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Cassandri M et al. Cell Death Discov 2024; 10 (1):351 HDAC3 genetic and pharmacologic inhibition radiosensitizes fusion positive rhabdomyosarcoma by promoting DNA double-strand breaks.
Xu Y et al. Cell Biosci 2023; 13 (1):7 Activation of goblet cell Piezo1 alleviates mucus barrier damage in mice exposed to WAS by inhibiting H3K9me3 modification.
Application : WB
Reactivity : Human, Mouse
Richter HJ et al. Proc Natl Acad Sci U S A 2022; 119 (33):e2205276119 Balanced control of thermogenesis by nuclear receptor corepressors in brown adipose tissue.
Chen CY et al. Front Oncol 2020; 10:216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.
Application : WB
Reactivity : Human
Huang D et al. Nat Immunol 2018; (Epub) NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.
Application : ChIP assay
Reactivity : Human
Lee JY et al. Front Pharmacol 2016; 7:81 Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells.
Application : WB
Reactivity : Human
Yuan H et al. Acta Physiol (Oxf) 2014; Exercise increases the binding of MEF2A to the Cpt1b promoter in mouse skeletal muscle.
Application : ChIP assay
Reactivity : Mouse
Chuang C et al. PLoS One 2013; 8 (9):e73841 NudC deacetylation regulates mitotic progression.
Application : IP
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169