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HDAC3 antibody [C3], C-term

Anti-HDAC3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Anti-HDAC3 antibody [C3], C-term used in ChIP assay (ChIP assay). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109679
Anti-HDAC3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679
Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

Cat No. GTX109679

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , *FaDu

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Histone Deacetylase 3,Hd3,Rpd3,Rpd3-2,Hdac3

Cellular Localization

Nucleus

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HDAC3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX109679

GTX109679 IP Image

HDAC3 antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 μg of HDAC3 antibody (GTX109679) 10% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody (GTX109679) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [C3], C-term used in ChIP assay (ChIP assay). GTX109679

GTX109679 ChIP assay Image

HDAC3 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of HDAC3 antibody (GTX109679) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Anti-HDAC3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109679

GTX109679 ICC/IF Image

HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-HDAC3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109679

GTX109679 ICC/IF Image

HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:400.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-HDAC3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109679

GTX109679 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HDAC3(GTX109679) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

Sample(30 µg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX109679 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC3 antibody [C3], C-term used in Western Blot (WB). GTX109679

GTX109679 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

HDAC3 antibody [C3], C-term Cat No. GTX109679

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-Dec-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : FADU cells
Amount used : 15μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169