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HDAC6 antibody

Cat No. GTX12173

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse
Package
50 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
IP 5-10 μg
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Product Note

Anti-Histone Deacetylase 6 (HDAC6) recognizes human and mouse HDAC6 (~134 kDa). Detection of HDAC6 by immunoblotting is specifically inhibited with the immunizing peptide. Additional nonspecific bands of approx. 50 kDa may be detected in various extract preparations.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding to amino acid residues 1199-1213 of human HDAC6. The corresponding sequence in mouse differs by one amino acid.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone deacetylase 6 , CPBHM , HD6 , JM21 , PPP1R90

Cellular Localization

Nucleus,Cytoplasm,Cell projection, dendrite

Background

Regulation of gene expression is mediated by several mechanisms. Among them are DNA methylation, ATP dependent chromatin remodeling, and post-translational modifications of histones, such as the dynamic acetylation and deacetylation of epsilon-amino groups of lysine residues present in the tail of core histones. The enzymes responsible for this reversible acetylation/deacetylation process are histone acetyltransferases(HATs) and histone deacetylases (HDACs), respectively. HATs act as transcriptional coactivators and HDACs are part of transcriptional corepressor complexes. Mammalian HDACs can be divided into three classesaccording to sequence homology. Class I consists of the yeast Rpd3-like proteins HDAC1, HDAC2, HDAC3 and HDAC8. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. Class III consists of the yeast Sir2-like proteins. Whereas class I HDACs are ubiquitously expressed, most class II HDACs are tissue-specific. Class II HDACs are larger than those of class I and their catalytic domain is located in the carboxy terminal half of the protein. HDAC6 is a unique deacetylase among class II members in that it contains a duplicate of the catalytic domain in its NH2 terminus as well. The deacetylase activity of class II HDACs is regulated by subcellular localization. Endogenous HDAC6 is predominantly cytoplasmic where it associates with microtubules and functions as an alpha-tubulin deacetylase. HDAC6 contains a conserved zinc finger motif that is probably involved in the regulation of ubiquitination.

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379