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HIF2 alpha antibody [N1N3]

Anti-HIF2 alpha antibody [N1N3] used in Western Blot (WB). GTX103707

Cat No. GTX103707

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ChIP assay

Reactivity

Human, Zebrafish
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

HepG2 (1% O2 treatment for 24hr) , *HT1080 , *786-0 , *FADU

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

endothelial PAS domain protein 1 , ECYT4 , HIF2A , HLF , MOP2 , PASD2 , bHLHe73

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF2 alpha antibody [N1N3] used in Western Blot (WB). GTX103707

GTX103707 WB Image

HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract (1% O2 treatment for 24 hr)
7.5% SDS-PAGE
HIF2 alpha antibody (GTX103707) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

HIF2 alpha antibody [N1N3] Cat No. GTX103707

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 25-Nov-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT1080,786-0 cells
Amount used : 50μg
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : A: HT1080 cells,B: 786-0 cells
Figure Note : Dr Norazizah Shafee, Universiti Putra Malaysia
Date : Anonymous submitted on 22-Dec-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : FADU cells
Amount used : 40μg
Blocking : 4% BSA, 25°C, 3Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169