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HIF2 alpha antibody [ep190b]

Anti-HIF2 alpha antibody [ep190b] used in Flow cytometry (FACS). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in IHC (Paraffin sections) (IHC-P). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

Cat No. GTX30123

Host

Mouse

Clonality

Monoclonal

Clone Name

ep190b

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Gel supershift assays, IHC

Reactivity

Human, Mouse, Rat, Hamster
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/ml
ICC/IF Assay dependent
IHC-P 1:150 - 1:300
IHC-Fr Assay dependent
FACS 1:400
IP Assay dependent
ELISA 1:100 - 1:2000
ChIP assay Assay dependent
Gel supershift assays Assay dependent
IHC 1:150 - 1:300
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Specificity/Sensitivity

This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

endothelial PAS domain protein 1 , ECYT4 , HIF2A , HLF , MOP2 , PASD2 , bHLHe73

Cellular Localization

Nucleus,Nucleus speckle

Background

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF2 alpha antibody [ep190b] used in Flow cytometry (FACS). GTX30123

GTX30123 FACS Image

FACS analysis of HepG2 cells using GTX30123 HIF2 alpha antibody [ep190b]. M1 is defined by unstained cells.
Purple : primary antibody
Dilution : 1:400

Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

GTX30123 WB Image

WB analysis of multiple samples using GTX30123 HIF2 alpha antibody [ep190b].
Lane 1 and 5: HepG2 without Cobalt (II) Chloride
Lane 2 and 6: HepG2 with Cobalt (II) Chloride
Lane 3 and 7: HepG2 normoxic
Lane 4 and 8 : HepG2 hypoxic
Dilution : 1-2 μg/ml

Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

GTX30123 WB Image

WB analysis of HeLa cell lysate using GTX30123 HIF2 alpha antibody [ep190b].

Anti-HIF2 alpha antibody [ep190b] used in IHC (Paraffin sections) (IHC-P). GTX30123

GTX30123 IHC-P Image

IHC-P analysis of human heart tissue using GTX30123 HIF2 alpha antibody [ep190b].

Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

GTX30123 WB Image

WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b].
Lane 1: normoxic A549 lysate control
Lane 2: hypoxic A549 lysate

Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

GTX30123 WB Image

VHL disease associated mutations demonstrate a graded amount of HIF Regulation.A. Anti-HA immunoblot for expression of HA-tagged human pVHL in transgenic 786-0 clones. Whole-cell protein extracts were prepared from 786-0 clones deficient for VHL expression (Vector) or modestly expressing wild-type (WT) or missense (Y112H, R167Q) mutant HA-tagged human pVHL. B. Anti-HIF-2α immunoblot for HIF stabilization in 786-0 clones. Whole-cell protein extracts were prepared from VHL-deficient 786-0 cells or WT or mutant HA-pVHL-rescued 786-0 cells incubated in the presence or absence of the hypoxia mimetic CoCl2 for 24 hours. Ku80 immunoblot was used as a control for equal loading.

REFERENCE

REVIEW

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Package List Price ($)
$ 369