PRODUCT
Summary
HIF2 alpha antibody recognizes HIF2 alpha / EPAS1 protein, a transcription factor (predicted molecular weight of 97 kDa) induced by hypoxic conditions. It forms a heterodimer with HIF1 beta / ARNT to drive a specific gene expression profile in various physiological and pathological processes that occur in the context of hypoxia.
APPLICATION
Application Note
Recommended Starting Dilutions:
For WB: Use at a dilution of 1:100-1:3000. Recognizes a band at 118kDa.
For IHC: Use at a dilution of 1:100.
For ICC/IF: Use at a dilution of 1:100-1:1000.
Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
HepG2 (1% O2 treatment for 24hr and using Urea buffer) , Myc-DDK human HIF2A-transfected 293T
Product Note
Specific for HIF-2 alpha/EPAS. Does not cross react with HIF-1 alpha.
Predict Reactivity
Human (100%)| Mouse (100%)| Rat (86%)| Bovine (80%)| Chicken (80%)| Pig (93%)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
A peptide derived from the mouse/human HIF2 alpha.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_370290
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
endothelial PAS domain protein 1 , HIF-2alpha , HIF2A , HLF , HRF , MOP2 , bHLHe73
Background
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells. (1)
Database
Research Area
DATA IMAGES
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GTX30114 ICC/IF Image
HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200.Blue: Hoechst 33342 staining.
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GTX30114 ICC/IF Image
HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis.Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200.Blue: Hoechst 33342 staining.
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GTX30114 WB Image
GTX30114 utilized in western blot with PC12 nuclear extracts, 1:1,000, 5 second exposure.
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GTX30114 WB Image
Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using GTX30114
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GTX30114 WB Image
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 0.01 O2 treatment for 24h) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX30114 ICC/IF Image
Immunocytochemistry/Immunofluorescence: HIF-2 alpha Antibody (GTX30114) - Detection of HIF-2 Alpha (Green) in RCC4 cells using GTX30114. Nuclei (Blue) are counterstained with Hoechst 33258.
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GTX30114 WB Image
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Non-transfected (-) and HIF2A-transfected (+, including Myc-DDK-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX30114 WB Image
Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis on normoxic and hypoxic nuclear rat cell lysates.
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GTX30114 IHC Image
Immunohistochemistry: HIF-2 alpha Antibody (GTX30114) - Hif-2 alpha immunoreactivity in human cardiac myocytes
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GTX30114 WB Image
The data was published in the journal Cell Death Dis in 2014. PMID: 25010988
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GTX30114 WB Image
The data was published in the journal Oncotarget in 2017. PMID: 29137374
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REFERENCE
REVIEW
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