Western blotting of functional recombinant full-length HIV-1 reverse transcriptase by using anti-HIV-1 Reverse Transriptase antibody. lane 1: 40 ng/lane 2: 20 ng/lane 3: 4 ng/lane 4: 2 ng/lane Anti-HIV-1 RT antibody was used at 1/2,000 dilution. As second antibody, goat anti-rabbit IgG antibody conjugated with HRP was used at 1/5,000 dilution. ECL system was used.

Detection of HIV-1 reverse transcriptase in the extract of HIV-1 infected cells by Western blotting using anti-HIV transcriptase antibody Lnae 1: Extract of MT4 cells Lane 2: Extract of MT4 cells infected with HIV-1 (LAI strain) The antiserum was diluted 2,500 fold before use.