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HLA-A antibody [N1C2]

Anti-HLA-A antibody [N1C2] used in Immunoprecipitation (IP). GTX114080
Anti-HLA-A antibody [N1C2] used in Western Blot (WB). GTX114080
Anti-HLA-A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114080

Cat. No. GTX114080

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HLA-A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164325

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class I, A , HLAA

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-A antibody [N1C2] used in Immunoprecipitation (IP). GTX114080

GTX114080 IP Image

HLA-A antibody [N1C2] immunoprecipitates HLA-A protein in IP experiments.
IP samples: A549 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of HLA-A protein by 4 μg HLA-A antibody [N1C2] (GTX114080)
10 % SDS-PAGE
The immunoprecipitated HLA-A protein was detected by HLA-A antibody [N1C2] (GTX114080) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-HLA-A antibody [N1C2] used in Western Blot (WB). GTX114080

GTX114080 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-A antibody [N1C2] (GTX114080) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HLA-A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114080

GTX114080 IHC-P Image

HLA-A antibody [N1C2] detects HLA-A protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
HLA-A stained by HLA-A antibody [N1C2] (GTX114080) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Rashid MU et al. Front Microbiol 2022; 13 : 994512 Temporal proteomic analyses of human lung cells distinguish high pathogenicity influenza viruses and coronaviruses from low pathogenicity viruses.
Application : WB
Reactivity : Human
Sher AA et al. Viruses 2022; 15 (1) HLA-A, HSPA5, IGFBP5 and PSMA2 Are Restriction Factors for Zika Virus Growth in Astrocytic Cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169