HLA-A antibody [N1C2]

Cat. No. GTX114080

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all HLA-A products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent
IP	1:100-1:500

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164325

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

major histocompatibility complex, class I, A , HLAA

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3105 HLA-A
UniProt: P30443
UniProt: P04439

Research Area

DATA IMAGES

GTX114080 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-A antibody [N1C2] (GTX114080) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114080 IHC-P Image

HLA-A antibody [N1C2] detects HLA-A protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
HLA-A stained by HLA-A antibody [N1C2] (GTX114080) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114080 IP Image

HLA-A antibody [N1C2] immunoprecipitates HLA-A protein in IP experiments.
IP samples: A549 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of HLA-A protein by 4 μg HLA-A antibody [N1C2] (GTX114080)
10 % SDS-PAGE
The immunoprecipitated HLA-A protein was detected by HLA-A antibody [N1C2] (GTX114080) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty