HLA-A antibody [N1C2] detects HLA-A protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
HLA-A stained by HLA-A antibody [N1C2] (GTX114080) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-A antibody [N1C2] (GTX114080) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HLA-A antibody [N1C2] immunoprecipitates HLA-A protein in IP experiments.
IP samples: A549 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of HLA-A protein by 4 μg HLA-A antibody [N1C2] (GTX114080)
10 % SDS-PAGE
The immunoprecipitated HLA-A protein was detected by HLA-A antibody [N1C2] (GTX114080) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]