APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
1:100-1:500 |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.76 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HLA-A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11164325
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
major histocompatibility complex, class I, A , HLAA
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX114080 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-A antibody [N1C2] (GTX114080) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114080 IHC-P Image
HLA-A antibody [N1C2] detects HLA-A protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human tonsil.HLA-A stained by HLA-A antibody [N1C2] (GTX114080) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX114080 IP Image
HLA-A antibody [N1C2] immunoprecipitates HLA-A protein in IP experiments. IP samples: A549 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of HLA-A protein by 4 μg HLA-A antibody [N1C2] (GTX114080) 10 % SDS-PAGE The immunoprecipitated HLA-A protein was detected by HLA-A antibody [N1C2] (GTX114080) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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REFERENCE
There are currently no references for HLA-A antibody [N1C2] (GTX114080). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HLA-A antibody [N1C2] (GTX114080). Be the first to share your experience with this product.