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HMBS antibody

Anti-HMBS antibody used in Immunoprecipitation (IP). GTX02363
Anti-HMBS antibody used in Western Blot (WB). GTX02363

Cat No. GTX02363

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IP 1-5 μl/mg of lysate
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Observed MW

46 kDa. The observed M.W. is based on the following publication. PMID: 19021769

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.0), 0.03% Proclin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E. coli-derived Human HMBS fragment

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hydroxymethylbilane synthase , PBG-D , PBGD , PORC , UPS

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HMBS antibody used in Immunoprecipitation (IP). GTX02363

GTX02363 IP Image

IP analysis of K562 whole cell lysate using GTX02363 HMBS antibody.
IP antibody : 4 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
Immunomagnetic beads Protein A/G : 60 μg

Anti-HMBS antibody used in Western Blot (WB). GTX02363

GTX02363 WB Image

WB analysis of various samples using GTX02363 HMBS antibody.
Lane A : HepG2 whole cell lysate
Lane B : HeLa whole cell lysate
Lane C : U937 whole cell lysate
Lane D : K562 whole cell lysate
Dilution : 1:500
Loading : 30 μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 339