APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , H1299 , HCT116
Predict Reactivity
Mouse, Rat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGA1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
HMG-R antibody, HMGA1A antibody, HMGIY antibody, MGC12816 antibody, MGC4242 antibody, MGC4854 antibody, HMGA1 antibody, high mobility group protein HMG-I/HMG-Y antibody, high-mobility group (nonhistone chromosomal) protein isoforms I and Y antibody, high mobility group protein R antibody, HMG-I(Y) antibody, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y antibody, high mobility group protein A1 antibody, high mobility group AT-hook 1 antibody
Cellular Localization
Nucleus
Background
This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100307 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody [N1N3] (GTX100307) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
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GTX100307 WB Image
HMGA1 antibody [N1N3] detects HMGA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody [N1N3] (GTX100307) diluted at 1:500.
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