Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (GTX629478) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HMGA2 antibody [GT763] detects HMGA2 protein at on SkHep1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1 xenograft.
HMGA2 antibody [GT763] (GTX629478) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGA2 protein stained by HMGA2 antibody [GT763] (GTX629478) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

HMGA2 antibody [GT763] detects HMGA2 protein at on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
HMGA2 antibody [GT763] (GTX629478) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
HMGA2 stained by HMGA2 antibody [GT763] (GTX629478) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

HMGA2 antibody [GT763] detects HMGA2 protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
15% SDS-PAGE
HMGA2 antibody [GT763] (GTX629478) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (GTX629478) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (GTX629478) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HMGA2 antibody [GT763] detects HMGA2 protein on C2C12 by immunohistochemical analysis.
Sample: Paraffin-embedded C2C12.
HMGA2 antibody [GT763] (GTX629478) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody [GT763] (GTX629478).
Western blot analysis was performed using HMGA2 antibody [GT763] (GTX629478) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HMGA2 stained by HMGA2 antibody [GT763] (GTX629478) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min