HMGB1 antibody [GT348] detects HMGB1 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
HMGB1 antibody [GT348] (GTX628835) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HMGB1 antibody [GT348] detects HMGB1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 protein stained by HMGB1 antibody [GT348] (GTX628835) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT348] (GTX628835) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT348] (GTX628835) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT348] (GTX628835) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HMGB1 antibody [GT348] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT348] (GTX628835) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

HMGB1 antibody [GT348] detects HMGB1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT348] (GTX628835) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HMGB1 antibody [GT348] detects HMGB1 protein at nucleus on mouse lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
HMGB1 antibody [GT348] (GTX628835) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HMGB1 antibody [GT348] detects HMGB1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT348] (GTX628835) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HMGB1 antibody [GT348] detects HMGB1 protein at nucleus on mouse lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
HMGB1 antibody [GT348] (GTX628835) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HMGB1 antibody [GT348] detects HMGB1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT348] (GTX628835) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.