HMGB1 antibody [GT412] detects HMGB1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
HMGB1 antibody [GT412] (GTX629400) dilution: 1:1000

HMGB1 antibody [GT412] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT412] (GTX629400) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] (GTX629400) diluted at 1:3000.

HMGB1 antibody [GT412] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] (GTX629400) diluted at 1:1000.

HMGB1 antibody [GT412] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT412] (GTX629400) diluted at 1:500.
Red: GFAP, a glia cell marker, stained by GFAP antibody (GTX27260) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

HMGB1 antibody [GT412] detects HMGB1 protein at nucleus in human ovarian cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
HMGB1 antibody [GT412] (GTX629400) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] (GTX629400) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.