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HMGB1 antibody [HL2059]

Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963
Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963
Anti-HMGB1 antibody [HL2059] used in ELISA (ELISA). GTX637963
Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963
Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963
Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963
Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963
Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963
Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963
Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963

Cat. No. GTX637963

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2059

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Product Note

This antibody is specific for human HMGB1 protein, and it does not cross react with human HMGB2 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus,Cytoplasm,Secreted,Cell membrane,Endosome

Background

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963

GTX637963 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963

GTX637963 ICC/IF Image

HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-HMGB1 antibody [HL2059] used in ELISA (ELISA). GTX637963

GTX637963 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, human HMGB1 protein (1000-15.63 ng/mL). Coated protein was probed with HMGB1 antibody [HL2059] (GTX637963) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963

GTX637963 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963

GTX637963 IHC-P Image

HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963

GTX637963 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:300000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963

GTX637963 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963

GTX637963 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963

GTX637963 IHC-P Image

HMGB1 antibody [HL2059] detects HMGB1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963

GTX637963 ICC/IF Image

HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

REFERENCE

There are currently no references for HMGB1 antibody [HL2059] (GTX637963). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HMGB1 antibody [HL2059] (GTX637963). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations