APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1
Cellular Localization
Nucleus,Cytoplasm,Secreted,Cell membrane,Endosome
Background
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/templates/default/js/lightbox/images/loading.gif "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
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GTX637963 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637963 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637963 ELISA Image
Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, human HMGB1 protein (1000-15.63 ng/mL). Coated protein was probed with HMGB1 antibody [HL2059] (GTX637963) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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GTX637963 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637963 IHC-P Image
HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637963 ICC/IF Image
HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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REFERENCE
There are currently no references for HMGB1 antibody [HL2059] (GTX637963). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HMGB1 antibody [HL2059] (GTX637963). Be the first to share your experience with this product.
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_44935_20230210_WB_shRNA_watermark_23021401_300.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_T-44886_20221209_WB_M_R_22121123_430.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in ELISA (ELISA). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_T-44886_20220113_ELISA_Indirect_23011618_221.webp "Anti-HMGB1 antibody [HL2059] used in ELISA (ELISA). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_44935_20230203_WB_23020621_399.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_T-44886_20221230_IHC-P_multiple_M_23020621_380.webp "Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_T-44886_20230203_ICC_IF_23021401_804.webp "Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230210_WB_shRNA_watermark_23021401_300.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20221209_WB_M_R_22121123_430.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in ELISA (ELISA). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20220113_ELISA_Indirect_23011618_221.webp "Anti-HMGB1 antibody [HL2059] used in ELISA (ELISA). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230203_WB_23020621_399.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20221230_IHC-P_multiple_M_23020621_380.webp "Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20230203_ICC_IF_23021401_804.webp "Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963")