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HMGB1 antibody

KO/KD
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32656
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32656
Anti-HMGB1 antibody used in Western Blot (WB). GTX32656
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32656
Anti-HMGB1 antibody used in Western Blot (WB). GTX32656

Cat. No. GTX32656

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($409)
See all HMGB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human HMGB1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus,Cytoplasm,Secreted,Cell membrane,Endosome

Background

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32656

GTX32656 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX32656 HMGB1 antibody.
Dilution : 1:100

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32656

GTX32656 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX32656 HMGB1 antibody.
Dilution : 1:100

Anti-HMGB1 antibody used in Western Blot (WB). GTX32656

GTX32656 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32656 HMGB1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32656

GTX32656 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX32656 HMGB1 antibody.
Dilution : 1:100

Anti-HMGB1 antibody used in Western Blot (WB). GTX32656

GTX32656 WB Image

WB analysis of MCF7 cell lysate using GTX32656 HMGB1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409