Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129469) diluted at 1:1000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129469) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HMGB2 antibody detects HMGB2 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HMGB2 antibody (GTX129469) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HMGB2 antibody detects HMGB2 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB2 protein stained by HMGB2 antibody (GTX129469) diluted at 1:1000.
Blue: Hoechst 33342 staining.

HMGB2 antibody detects HMGB2 protein by Western blot analysis.
A. 30 μg 293T
B. 30 μg A431
C. 30 μg HeLa
D. 30 μg HepG2
12 % SDS-PAGE
HMGB2 antibody (GTX129469) dilution: 1:1000