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HMW Kininogen antibody

Anti-HMW Kininogen antibody used in Western Blot (WB). GTX100833
Anti-HMW Kininogen antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100833
Anti-HMW Kininogen antibody used in IHC (Paraffin sections) (IHC-P). GTX100833

Cat. No. GTX100833

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Kininogen 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

Molt-4

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMW Kininogen. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950634

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kininogen 1 , BDK , BK , HMWK , KNG

Background

High molecular weight kininogen (HMWK) plays an important role in assembly of the plasma kallikrein (see MIM 147910)-kinin system. The KNG1 gene generates both HMWK and low molecular weight kininogen (LMWK) through alternative splicing. Both HMWK and LMWK contain an identical heavy chain consisting of protein domains 1, 2, and 3. However, HMWK contains a 56-kD light chain that consists of domains 5 and 6H, whereas LMWK contains a unique 4-kD light chain that consists of domain 5L. In both proteins, the heavy and light chains are linked by domain 4, which contains the bradykinin (BK) nonapeptide. BK, which is released by plasma kallikrein, is a potent inflammatory mediator that causes vasodilation and enhanced capillary permeability, induces pain, and stimulates production of nitric oxide and prostacyclin (see MIM 601699) from endothelial cells. During vascular damage, BK stimulates smooth muscle proliferation and intimal hypertrophy. Release of BK from HMWK generates a 2-chain HMWK, termed HMWKa, containing the heavy and light chains joined by a disulfide bond (Merkulov et al., 2008 [PubMed 18000168]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HMW Kininogen antibody used in Western Blot (WB). GTX100833

GTX100833 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX100833 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMW Kininogen antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100833

GTX100833 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Kininogen-1(GTX100833) antibody at 1:200 dilution.

Anti-HMW Kininogen antibody used in IHC (Paraffin sections) (IHC-P). GTX100833

GTX100833 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Kininogen 1(GTX100833) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Droll SH et al. Rapid Commun Mass Spectrom 2019; (Epub) Differential Processing of High-Molecular-Weight Kininogen during Normal Pregnancy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169