*Optimal dilutions/concentrations should be determined by the researcher.
|1-2μg/ml for 30 minutes at RT
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.
Epitope is localized between aa 383-447 of human hsp60.
We do not recommend use of this product for Spinach,Bacteria,Helminths.
10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant human HSP60 protein
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CPN60 , GROEL , HLD4 , HSP60 , HSP65 , HSPD1 , HuCHA60 , SPG13 , heat shock protein family D (Hsp60) member 1 , Heat Shock Protein 60
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
GTX34786 WB Image
WB analysis of HeLa cell lysate using GTX34786 HSP60 antibody [SPM253].
GTX34786 IHC-P Image
IHC-P analysis of human bladder carcinoma tissue using GTX34786 HSP60 antibody [SPM253].
GTX34786 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX34786 HSP60 antibody [SPM253].
GTX34786 ICC/IF Image
ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34786 HSP60 antibody [SPM253].
Green : Primary antibody
Red : nucleus