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HSP60 antibody [SPM253]

Anti-HSP60 antibody [SPM253] used in IHC (Paraffin sections) (IHC-P). GTX34786
Anti-HSP60 antibody [SPM253] used in IHC (Paraffin sections) (IHC-P). GTX34786

Cat No. GTX34786

Host Mouse
Clonality Monoclonal
Clone Name SPM253
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Rabbit, Sheep, Drosophila, Bovine, Dog, Hamster, Chicken, Pig, Monkey, Xenopus laevis
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.25-0.5ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srp?s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human HSP60 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family D (Hsp60) Member 1,Cpn60,Groel,Hld4,Hsp-60,Hsp60,Hsp65,Hucha60,Spg13,Hspd1

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES
Anti-HSP60 antibody [SPM253] used in IHC (Paraffin sections) (IHC-P). GTX34786

GTX34786 IHC-P Image

Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with HSP60 Monoclonal Antibody (SPM253)

Anti-HSP60 antibody [SPM253] used in IHC (Paraffin sections) (IHC-P). GTX34786

GTX34786 IHC-P Image

Formalin-fixed, paraffin-embedded human Pancreas stained with HSP60 Monoclonal Antibody (SPM253).