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HSP60 antibody [SPM253]

Anti-HSP60 antibody [SPM253] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34786
Anti-HSP60 antibody [SPM253] used in IHC (Paraffin sections) (IHC-P). GTX34786
Anti-HSP60 antibody [SPM253] used in IHC (Paraffin sections) (IHC-P). GTX34786
Anti-HSP60 antibody [SPM253] used in Western Blot (WB). GTX34786

Cat. No. GTX34786

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM253

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Drosophila, Bovine, Dog, Hamster, Chicken, Pig, Monkey, Xenopus laevis
Package
100 μg ($529)
See all HSP60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Product Note

Epitope is localized between aa 383-447 of human hsp60.
We do not recommend use of this product for Spinach,Bacteria,Helminths samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HSP60 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CPN60 , GROEL , HLD4 , HSP60 , HSP65 , HSPD1 , HuCHA60 , SPG13 , heat shock protein family D (Hsp60) member 1 , Heat Shock Protein 60

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody [SPM253] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34786

GTX34786 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34786 HSP60 antibody [SPM253].
Green : Primary antibody
Red : nucleus

Anti-HSP60 antibody [SPM253] used in IHC (Paraffin sections) (IHC-P). GTX34786

GTX34786 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX34786 HSP60 antibody [SPM253].

Anti-HSP60 antibody [SPM253] used in IHC (Paraffin sections) (IHC-P). GTX34786

GTX34786 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX34786 HSP60 antibody [SPM253].

Anti-HSP60 antibody [SPM253] used in Western Blot (WB). GTX34786

GTX34786 WB Image

WB analysis of HeLa cell lysate using GTX34786 HSP60 antibody [SPM253].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529