GeneTex
United States (US)

Hdac3 antibody

GTX124349_40436_IHC-Wm_Z_18121410_970.jpg
GTX124349_40436_IHC_Z_18121410_713.jpg
GTX124349_40436_WB_Z_18121410_184.jpg

Cat No. GTX124349

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Wm
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Adult zebrafish , zebrafish embryo , Ubiquitous express
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Hdac3

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Histone Deacetylase 3,Zgc:55927,Hdac3

Cellular Localization

Nucleus

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

Database

Research Area

DATA IMAGES
GTX124349_40436_IHC-Wm_Z_18121410_970.jpg

GTX124349 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using hdac3 (GTX124349) antibody at 1:200 dilution.

GTX124349_40436_IHC_Z_18121410_713.jpg

GTX124349 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using hdac3 (GTX124349) antibody at 1:300 dilution.

GTX124349_40436_WB_Z_18121410_184.jpg

GTX124349 WB Image

Sample (30 ug of whole cell lysate)
A: whole zebrafish
10% SDS PAGE
GTX124349 diluted at 1:500

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf