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Hepatitis B Virus X Protein antibody [X36C]

Anti-Hepatitis B Virus X Protein antibody [X36C] used in Western Blot (WB). GTX22741

Cat No. GTX22741

Host Mouse
Clonality Monoclonal
Clone Name X36C
Isotype IgG1
Application WB, ICC/IF, IP, ELISA, ChIP assay, IHC
Reactivity Hepatitis B virus
Package
100 μg ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/ml
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody is not capable of immunoprecipitating HBx complexed with HBx interacting protein (XIP).
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Hepatitis B virus

Immunogen

Baculovirus expressed recombinant HBx.

Purification

Purified by HPLC

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

The hepatitis B virus is an important cause of acute and chronic liver disease. It is also the most common form of parenterally transmitted viral hepatitis. The virus is estimated to affect some 350 million people worldwide. Immunization against hepatitis B is an important component of preventative medicine and is offered to all children in approximately 30 countries. Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and infection with HBV is usually more serious than infection with hepatitis A virus. In some individuals the infection is not cleared and followed by persistent intracellular viral carriage that can lead to the development of hepatocelular carcinomas. There are currently 400 million HBV carriers worldwide making HBV one of the most common human pathogens.

Research Area

DATA IMAGES
Anti-Hepatitis B Virus X Protein antibody [X36C] used in Western Blot (WB). GTX22741

GTX22741 WB Image

Non-transfected (–) and transfected (+) Huh7 whole cell extracts (25 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hepatitis B Virus X Protein antibody [X36C] (GTX22741) diluted at 1:1000.

REFERENCE
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REVIEW

Hepatitis B Virus X Protein antibody [X36C] Cat No. GTX22741

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 12-Jan-2016
Score :
Application Tested : WB
Sample Species : HBV
Sample : HBV infected Huh 7 cells
Amount used : 25μg
Blocking : 4% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Huh 7 cells, Lane 2: HBV infected Huh 7 cells